Labeling Chemistry Support
Explore our “Getting Started” and “Troubleshooting” sections for solutions to top inquiries and common problems. Browse through our "Guides and Tools" section to access a comprehensive portfolio of product-related support resources.
- Can anything be covalently labeled with a fluorophore, biotin, or linker?
- Which labeling chemistry should I use?
- My molecule has more than one reactive group on its surface. Can I control what sites are labeled?
- I labeled my protein with a fluorescent dye. Do I really need to do the ‘degree of labeling’ (DOL) determination?
- My molecule is insoluble in aqueous buffers. Can I still label my molecule?
- I labeled my protein with a fluorescent dye, but detect little or no fluorescence. Does this mean the labeling reaction was unsuccessful?
- I prefer not to use size exclusion chromatography to clean up my labeled protein after the labeling reaction. What other methods can be used for the post-reaction purification?
- I labeled my antibody with an amine-reactive dye, but it no longer binds to its antigen. What is causing this? Is there anything that can be done to prevent this from happening?
- My molecule precipitated during the labeling reaction. Why did this happen? What can be done to prevent this?
Guides and Tools
- Overview: Labeling Chemistry
- Overview: Labeling Amines
- Overview: Labeling Thiols
- Overview: Click-iT™ Labeling Technologies
- Overview: Antibody and Protein Labeling Kits
- Overview: Azide-Phosphine (Staudinger) Reaction Chemistry
- Overview: Carbodiimide Crosslinker Chemistry
- Overview: Carbonyl-reactive Crosslinker Chemistry
- Overview: SiteClick™ Antibody Labeling System
- Overview: Protein Labeling and Crosslinking
- Overview: Chemistry of Crosslinking
- Overview: Protein Crosslinking Applications
- Overview: Crosslinking and Protein Modification
- Overview: Molecular Probes™ School of Fluorescence
- Learning Center: Cell Analysis
- Cell Analysis Resource Library (useful technical, application, and other learning resources including application notes and methods)
- Citations: Crosslinker Application Reference Guide
- Guide: Fluorophores and Their Amine-Reactive Derivatives
- Guide: Thiol-Reactive Probes
- Guide: Click Chemistry
- Guide: Reagents for Modifying Aldehydes and Ketones
- Guide: Derivatization Reagents for Carboxylic Acids and Carboxamides
- Guide: Labeling Oligonucleotides and Nucleic Acids
- Guide: The Molecular Probes™ Handbook
- Images: Image Gallery
- Journal Articles: BioProbes Journal of Cell Biology Applications
- Newsletter: ProbesOnline™ Newsletter
- Protocols: Fluorescent Amine-reactive Labeling of IgM Antibodies
- Protocols: Cell Analysis Protocols
- Selection: Fluorophore Selection Guides
- Selection: Crosslinker Selection Guide
- Selection: R0 Values for Some Alexa Fluor Dyes
- Tech Note: Labeling Small Peptides with Amine-Reactive Dyes in Organic Solvents
- Tech Note: Fluorescence Quantum Yields (QY) and Lifetimes (τ) for Alexa Fluor Dyes
- Tutorials: Fluorescence and Flow Cytometry Tutorials
- Crosslinker Selection Tool: Provides quick access to customized lists of Thermo Scientific™ Pierce™ Crosslinkers that meet specific criteria, including target functional group, solubility, and cell membrane permeability.
- Labeling Chemistry Selection Tool: Quickly find the best reactive dye or label for your experiment. Whether you are labeling proteins and antibodies for immunofluorescence, nucleic acids for in situ hybridization, or lipids for membrane studies, this tool will help you find the right conjugation product.
- Fluorescence SpectraViewer: Plot and compare spectra, check spectral compatibility, and email the configuration to yourself or others in the lab.
- Antibody Search Tool
- Fluorescence SpectraViewer Mobile App: An app to plot and compare spectra, check spectral compatibility, and email the configuration to yourself or others in the lab. This mobile version of SpectraViewer acts as an extension of our online Fluorescence SpectraViewer.
- Alexa Fluor™ Selection Guide Widget: A widget designed to provide you with a concise and informative set of criteria for choosing the optimal Alexa Fluor™ dye for your antibody or protein labeling application.
- Cell Imaging Reagent Guide & Protocols App: An app designed to help you find fluorescent dyes, reagents, kits, and protocols for cell biology-related fluorescence microscopy applications. Also included are product guides for instrument calibration and setup, protein labeling, and secondary detection.
- 3D Cell Simulation & Cell Staining Tool (for iPad™ device): Learn about the cell and all its structures, rotate the cell 360 degrees, watch live cell videos, and virtually stain your own cell with Molecular Probes™ dyes.
- Gibco™ Cell Culture Companion: An app designed to capture and store vital experiment information as you count cells, perform cell culture calculations, and generate growth curves.
- DailyCalcs Science Calculator: An app designed to calculate molarity, dilution, molecular weight, and more. Version 3.0 now has eight calculators, including a new Specific Productivity Calculator for use with fed-batch cell culture processes and a Transfection calculator you can use to simplify transfection experimental setup.
- Flow Cytometry Reagent Guide and Protocols App: An app designed to help you find fluorescent reagents, kits, and protocols for cell biology–related flow cytometry applications. Also included are product guides for instrument calibration and setup, protein labeling, and secondary detection.
All the components necessary to develop the most appropriate assay for your research or discovery target of interest are available from us with our custom solutions. Additionally, our dedicated project managers will guide your project through every step of the process, and constantly keep you informed of our progress. Let us take the hassle out of your hands with a custom solution for you.
With a proven track record of over 18,000 custom antibody projects completed to date, our custom antibody development, purification, and screening services deliver the quality, consistency, and performance you need. Once your custom project is underway, you can track the real-time progress of your project via OpenProject™ software. Our manufacturing facilities are ISO 13485:2003 certified and registered with the FDA. We offer USDA, and OLAW accreditation standard, and AAALAC form accreditation by special request.
Every cell culture project is unique. We are committed to providing you with products and services customized to your research and production needs. Presenting the Gibco™ quality media you know and trust; customized to your specifications.
For Research Use Only. Not for use in diagnostic procedures.