Acclaim HPLC Columns | Thermo Fisher Scientific

Your go-to HPLC columns for separating complex mixtures

Thermo Scientific Acclaim HPLC Columns have a smaller pore size and higher surface area to deliver the high resolution you need for high-efficiency separations. Choose from a broad variety of functional groups including:

Conventional reversed-phase C18 and polar embedded phases
Mixed-mode phases
Application-specific phases

Thermo Fisher Scientific designed and manufactured all Acclaim columns to tight specifications to ensure you get consistent performance.

Acclaim Column Selection Guide

Acclaim HPLC column families

Acclaim reversed-phase & general purpose HPLC columns

Column	Use for	Data sheet
Acclaim 120 C18 columns	High efficiency reversed-phase columns for small molecule and complex mixtures separations, including LC-MS	Acclaim 120 C18 data sheet
Acclaim 120 C8 columns	Reversed-phase columns for small molecule with intermediate hydrophobic retention	Acclaim 120 C8 data sheet
Acclaim 300 C18 columns	Wide pore columns for LC-MS life science applications; protein and peptides	Acclaim 300 C18 data sheet
Acclaim Polar Advantage (PA) columns	Polar-embedded reversed-phase columns for separating polar and nonpolar analytes with 100% aqueous mobile phase	Acclaim PA data sheet
Acclaim Polar Advantage (PA2) columns	Polar-embedded columns with enhanced hydrolytic stability pH 1.5 – 10, excellent for polar and non-polar analytes with 100% aqueous mobile phase and LC-MS	Acclaim PA2 data sheet
Acclaim C30 columns	Very high hydrophobic retention; good for separating structurally related isomers	Acclaim C30 data sheet
Acclaim HILIC-10 columns	Designed for unique selectivity of hydrophilic molecules

Specifications for Acclaim reversed-phase & general-purpose HPLC columns
	Acclaim 120 C18	Acclaim 120 C8	Acclaim 300 C18	Acclaim PA	Acclaim PA2	Acclaim C30	Acclaim Phenyl-1	Acclaim HILIC-10
Bonded Phase	Octadecylsilane	Octylsilane	Octadecylsilane	Embedded sulfonamide	Embedded Amide	C30 alkylsilane	Phenyl	Proprietary
USP Type	L1	L7	L1	L60	L60	L62	L11	na
End-capped	Yes	Yes	Yes	Yes	Yes	Proprietary	Yes
Starting Material	Ultrapure silica	Ultrapure silica	Ultrapure silica	Ultrapure silica	Ultrapure silica	Ultrapure silica	Ultrapure silica	Ultrapure silica
Particle Shape	Spherical	Spherical	Spherical	Spherical	Spherical	Spherical	Spherical	Spherical
Particle Sizes	2.2 μm, 3 μm, 5 μm	2.2 μm, 3 μm, 5 μm	3 μm	3 μm, 5 μm	3 μm, 5 μm	3 μm, 5 μm	3 μm, 5 μm	3 μm, 5 μm
Average Pore Diameter	120 Å	120 Å	300 Å	120 Å	120 Å	200 Å	120 Å	120 Å
Surface Area	300 m²/g	300 m²/g	100 m²/g	300 m²/g	300 m²/g	200 m²/g	300 m²/g	300 m²/g
Total Carbon Content	18%	11%	7%	17%	17%

Acclaim mixed-mode HPLC columns

Column	Use for	Data sheet
Acclaim Trinity P1 columns	Reversed-phase, anion-exchange, and cation exchange functionalities For superior resolution of API and counter ions, as well as complex mixtures	Acclaim Trinity P1 data sheet
Acclaim Trinity P2 columns	HILIC, anion-exchange, and cation exchange functionalities For complex mixtures including separation pharmaceutical counterion; mono- and divalent cations and anions	Acclaim Trinity P2 data sheet
Acclaim Mixed-Mode WAX-1 columns	Reversed-phase and anion-exchange combined functionality For separating anionic molecules with powerful adjustable selectivity control	Acclaim Mixed-Mode WAX-1 data sheet
Acclaim Mixed-Mode WCX-1 columns	Reversed-phase and cation-exchange combined functionality For separating cationic molecules with powerful adjustable selectivity control	Acclaim Mixed-Mode WCX-1 data sheet
Acclaim Mixed-Mode HILIC-1 columns	Combined capability to operate in either reversed-phase or HILIC mode	Acclaim Mixed-Mode HILIC-1 data sheet

Specifications for Acclaim mixed-mode HPLC columns
	Acclaim Trinity P1	Acclaim Trinity P2	Acclaim Mixed-Mode WCX-1	Acclaim Mixed-Mode WAX-1	Acclaim Mixed-Mode HILIC-1
Functionality	Reversed-phase + weak anion exchange + strong cation exchange	HILIC + strong anion exchange + weak cation exchange	Reversed-phase + weak cation exchange	reversed-phase + weak anion exchange	Reversed-phase + HILIC
USP Type	n.a.	n.a.	L85	L78	n.a.
Starting Material	Ultra-pure silica	Ultra-pure silica	Ultra-pure silica	Ultra-pure silica	Ultra-pure silica
Particle Shape	Spherical	Spherical	Spherical	Spherical	Spherical
Particle Sizes	3 μm, 5 μm	3 μm, 5 μm	3 μm	3 μm, 5 μm	3 μm, 5 μm
Average Pore Diameter	120 Å	120 Å	300 Å	120 Å	120 Å
Surface Area	300 m²/g	300 m²/g	100 m²/g	300 m²/g	300 m²/g

Acclaim application-specific HPLC columns

Column	Use for	Data sheet
Acclaim Organic Acid HPLC Columns	Fast organic acid analysis; including both aromatic and aliphatic organic acids.	Acclaim Organic Acid data sheet
Acclaim Surfactant HPLC Columns	Designed for the separation of surfactants including anionic, non-ionic, cationic, and amphoteric surfactants in various matrices. The Surfactant Plus is compatible with LC-MS instrumentation	Acclaim Surfactant data sheet
Acclaim Surfactant Plus columns	Designed for the separation of surfactants including anionic, non-ionic, cationic, and amphoteric surfactants in various matrices. The Surfactant Plus column is compatible with LC-MS instrumentation	Acclaim Surfactant Plus data sheet
Acclaim Explosives E2 columns	Specialty columns to comply with EPA Method 8330; baseline resolution of all 14 explosive residues targeted by the EPA
Acclaim Trinity Q1 columns	Excellent for trace analysis of diquat and paraquat
Acclaim Carbonyl columns	Separation of DNPH derivatives of aldehydes and ketones; including regulated methods U.S. EPA 554, EPA 8315, EPA 1667, EPA TO-11, and CARB 1004.	Acclaim Carbonyl data sheet
Acclaim Carbamate columns	Separation of carbamate pesticides specified in U.S. EPA Method 531.2	Acclaim Carbamate data sheet
Acclaim AmG C18 columns	Reversed-phase separation of aminoglycoside antibiotics.	Acclaim AmG C18 data sheet

Specifications for Acclaim application-specific columns
	Acclaim Organic Acid	Acclaim Surfactant	Acclaim Surfactant Plus	Acclaim Explosives E2	Acclaim Carbonyl	Acclaim Carbamate	Acclaim AmG	Acclaim Trinity Q1
End-capped	Yes	Yes	Yes	Yes	Yes	Yes	Yes	Yes
Particle Shape	Spherical	Spherical	Spherical	Spherical	Spherical	Spherical	Spherical	Spherical
Particle Size	3 μm, 5 μm	3 μm, 5 μm	3 μm, 5 μm	2.2 μm, 3 μm, 5 μm	2.2 μm, 3 μm, 5 μm	2.2 μm, 3 μm, 5 μm	3 μm	3 μm, 5 μm
Average Pore Diameter	120 Å	120 Å	120 Å	300 Å	120 Å	120 Å	300 Å	300 Å
Surface Area	300 m²/g	300 m²/g	300 m²/g	100 m²/g	300 m²/g	300 m²/g	100 m²/g	100 m²/g

Acclaim size exclusion (SEC) columns

Column	Use for	Data sheet
Acclaim SEC-1000 and Acclaim SEC-300	Separation of water-soluble polymers using resin based columns.	Acclaim SEC columns data sheet

Specifications for Acclaim size exclusion columns (SEC)
	Acclaim SEC-300	Acclaim SEC-1000
Substrate	Hydrophilic polymethacrylate resin	Hydrophilic polymethacrylate resin
Particle Shape	Spherical	Spherical
Particle Size	5 μm	7 μm
Pore Size	300 Å (multi-pore)	1000 Å (multi-pore)
Separation range for PEO*	100 – 50,000 Daltons	1,000 – 1,000,000 Daltons
Exclusion limit for PEO*	50,000 – 150,000 Daltons	3,000,000 – 7,500,000 Daltons

Acclaim HPLC columns selection guide

Download the full selection guide PDF

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