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Overview
Enhanced low-flow LC-MS separation performance
Low-flow chromatography is ideal when detailed sample information is required from small sample volumes, such as in proteomics, metabolomics, or lipidomics research. Compared with analytical flow (>100 µL/min), low-flow LC-MS (<100 µL/min) typically provides a large increase in sensitivity. Low-flow columns—also known as nano, capillary, or micro columns—have a smaller diameter compared to analytical columns.
Thermo Scientific nano-, capillary-, and micro-flow columns offer excellent sensitivity and resolution in three easy-to-use formats:
- Thermo Scientific OptiSpray (U)HPLC Cartridges
- Thermo Scientific EASY-Spray UHPLC Columns
- Thermo Scientific nanoViper (U)HPLC Columns
All HPLC and UHPLC columns
- Popular small molecule HPLC and UHPLC column brands
- Biomolecule (U)HPLC columns
- Reversed phase (U)HPLC columns
- HILIC (U)HPLC columns
- Mixed-mode HPLC columns
- Application specific HPLC columns
- Normal phase HPLC columns
- Ion exclusion HPLC columns
- Preparative HPLC columns
- HPLC guard columns, cartridges, and accessories
Low-flow columns for reliable and robust data
Low-flow (U)HPLC column formats | ||
| OptiSpray Cartridges | EASY-Spray Columns | nanoViper Columns |
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Maximum low-flow LC-MS performance, reproducibility, and robustness
OptiSpray cartridges
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| Chose an OptiSpray cartridge column when: | What makes OptiSpray cartridges special? |
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OptiSpray µPAC Neo cartridges
Thermo Scientific OptiSpray µPAC Neo cartridges are ideally suited for applications requiring robust and reproducible performance across large sample sets.
| Catalog number | Product | Column volume (µL) | Column length (cm) | Flow rate range (nL/min) |
|---|---|---|---|---|
| OS-UPAC050NAN | µPAC Neo HPLC column | 1.5 | 50 | 100 – 750 |
| OS-UPAC005CAP | µPAC Neo high-throughput HPLC column | 1.5 | 5.5 | 100 – 3000 |
OptiSpray PepMap Neo cartridges
Choose Thermo Scientific PepMap Neo cartridges for versatility in analyzing the widest range of sample types.
| Catalog number | Product | Column ID (µm) | Column length (cm) | Flow rate range (nL/min) |
|---|---|---|---|---|
| OS75150PN | PepMap Neo column | 75 | 15 | 100 – 1500 |
| OS75500PN | PepMap Neo column | 75 | 50 | 100 – 700 |
OptiSpray infusion and source diagnostic cartridges
OptiSpray infusion and source diagnostics cartridges simplify mass spectrometer calibration and OptiSpray ion source diagnostics and troubleshooting, respectively, without the need to switch ion sources.
| Catalog number | Product | For use with |
|---|---|---|
| OSINFUSION | OptiSpray infusion cartridge | OptiSpray ion source |
| OSDIAGNOSTIC | OptiSpray source diagnostic cartridge | OptiSpray ion source |
Emitters
OptiSpray emitters are exchangeable electrospray ionization emitters that significantly extend the lifetime of OptiSpray cartridge columns. The tapered-tip emitter comes standard with capillary-flow cartridges, while the pulled-tip emitter is provided for nano-flow cartridges.
| Catalog number | Product | For use with |
|---|---|---|
| OSE1535T | OptiSpray tapered-tip emitter, 15 µm | OptiSpray cartridge columns |
| OSE0835P | OptiSpray pulled-tip emitter, 8 µm | OptiSpray cartridge columns |
Traps and accessories
For the best performance from your OptiSpray cartridge columns, consider investing in the following accessories.
| Catalog number | Product | Particle/pillar diameter (µm) | Column ID (µm) | Media bed length (mm) | For use with | Order |
|---|---|---|---|---|---|---|
| 174500 | PepMap Neo trap cartridge, 1500 bar | 5 | 300 | 5 | OptiSpray cartridge columns | Order now › |
| 164750 | PepMap nanotrap, 500 bar | 5 | 100 | 20 | OptiSpray cartridge columns | Order now › |
| 164535 | PepMap nanotrap, 500 bar | 3 | 75 | 20 | OptiSpray cartridge columns | Order now › |
| COL-TRPNANO16G1B2 | µPAC trapping column, 350 bar | 5 | 2000 | 10 | OptiSpray cartridge columns | Order now › |
| Catalog number | Product | For use with | Order |
|---|---|---|---|
| 174502 | Trap holder + nanoViper fittings kit, 1500 bar | nanoViper columns | Order now › |
Thermo Scientific OptiSpray Ion Source
Reliably achieve maximum ESI performance for proteomics, metabolomics, and lipidomics experiments across LC-MS systems, columns, and users of all experience levels.
EASY-Spray UHPLC columns
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| Choose an EASY-Spray column when: | What makes EASY-Spray columns special? |
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| Unique design provides high separation performance in an easy-to-use format for nano and capillary LC-MS analysis. Features include:
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Recommended EASY-Spray UHPLC columns by application
EASY-Spray PepMap Neo UHPLC columns
The Thermo Scientific EASY-Spray PepMap Neo UHPLC columns are perfect for bottom-up proteomics. Packed at higher pressure and rated to 1500 bar, they provide consistent column-to-column performance, long column lifetime, and excellent efficiency. These benefits are true at any pressure.
| Catalog number | Product | Particle size (µm) | Column ID (µm) | Column length (mm) | Order |
|---|---|---|---|---|---|
| ES75150PN | PepMap Neo columns | 2 | 75 | 150 | Order now › |
| ES75500PN | PepMap Neo columns | 2 | 75 | 500 | Order now › |
| ES75750PN | PepMap Neo columns | 2 | 75 | 750 | Order now › |
Also see: EASY-Spray PepMap HPLC columns
EASY-Spray MAbPac RP and Accucore C4 columns
Thermo Scientific MAbPac RP and Accucore C4 capillary columns are best suited for the characterization of intact proteins in top-down proteomics, clinical and anti-doping applications where sample amount is limited, or sensitivity is crucial.
| Catalog number | Product | Particle size (µm) | Column ID (µm) | Column length (mm) | Order |
|---|---|---|---|---|---|
| ES907 | MAbPac RP column | 4 | 150 | 150 | Order now › |
| ES911 | Accucore 150-C4 column | 2.6 | 75 | 150 | Order now › |
Traps and accessories
For the best performance from your EASY-Spray column, consider investing in the following accessories.
Traps
| Catalog number | Product | Particle size (µm) | Column ID (µm) | Media bed length (mm) | Trap length (mm) | Union type | Order |
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| 174500 | PepMap Neo Trap Cartridge, 3PK | 5 | 300 | 5 | N/A | N/A | Order now › |
| 164199 | PepMap nanotrap, 500 bar, 2PK | 5 | 100 | 20 | 150 | Nut/sleeve | Order now › |
| 164750 | PepMap nanotrap, 500 bar, 2PK | 5 | 100 | 20 | 150 | Double nanoViper | Order now › |
| 164535 | PepMap nanotrap, 500 bar, 2PK | 3 | 75 | 20 | 150 | Double nanoViper | Order now › |
| 164946 | PepMap nanotrap, 1200 bar, 2PK | 3 | 75 | 20 | 70 | Double nanoViper | Order now › |
| 164213 | PepMap nanotrap, 500 bar, 2PK | 5 | 200 | 20 | 150 | Nut/sleeve | Order now › |
PEEK tubing and trap holder
| Catalog number | Product | For use with | Order |
|---|---|---|---|
| 174501 | PEEK with nanoViper fittings, 30 µm X100 mm, 2PK, 1500 bar | Low-flow PepMap columns | Order now › |
| 174502 | Trap holder + nanoViper fittings kit, 1500 bar | Low-flow PepMap columns | Order now › |
nanoViper HPLC and UHPLC columns
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| Chose a nanoViper column when: | What makes nanoViper columns special? |
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| These stand-alone nano and capillary columns:
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Recommended nanoViper columns by application
µPAC Neo columns for bottom-up proteomics
µPAC Neo HPLC columns are ideally suited for applications requiring robust and reproducible performance across large sample sets.
| Catalog number | Product | Column volume (µL) | Column length (cm) | Flow rate range (nL/min) | Order |
|---|---|---|---|---|---|
| COL-NANO050NEOB | µPAC Neo HPLC column | 1.5 | 50 | 100 – 750 | Order now › |
| COL-NANO110NEOB | µPAC Neo HPLC column | 4.5 | 110 | 100 – 750 | Order now › |
| COL-CAPHTNEOB | High-throughput µPAC Neo HPLC column | 1.5 | 5.5 | 100 – 2500 | Order now › |
PepMap Neo columns for bottom-up proteomics
Single and Double nanoViper PepMap Neo columns are packed at a higher pressure and rated to 1500 bar and provide consistent column-to-column performance and excellent efficiency. In addition, they are ideal for use with the Thermo Scientific Vanquish Neo Tandem Direct Injection workflow.
| Catalog number | Product | Column ID (µm) | Column length (mm) | Flow rate range (nL/min) | Order |
|---|---|---|---|---|---|
| DNV75150PN | Double nanoViper PepMap Neo column | 75 | 150 | 100 – 1500 | Order now › |
| DNV75500PN | Double nanoViper PepMap Neo column | 75 | 500 | 100 – 700 | Order now › |
| DNV75750PN | Double nanoViper PepMap Neo column | 75 | 750 | 100 – 500 | Order now › |
| DNV150150PN | Double nanoViper PepMap Neo column | 150 | 150 | 1000 – 4000 | Order now › |
| SNV75750PN | Single nanoViper PepMap Neo column | 75 | 750 | 100 – 500 | Order now › |
| SNV150150PN | Single nanoViper PepMap Neo column | 150 | 150 | 1000 – 4000 | Order now › |
Also available: Single nanoViper PepMap C18 HPLC columns
HPLC columns for top-down proteomics
The Thermo Scientific MAbPac RP column is best suited for the characterization of intact proteins in top-down proteomics, clinical, and anti-doping applications where sample amount is limited, or sensitivity is crucial.
| Catalog number | Product | Column ID (µm) | Column length (mm) | Flow rate range (nL/min) | Order |
|---|---|---|---|---|---|
| 164947 | MAbPac RP column | 150 | 150 | 1000 – 3000 | Order now › |
UHPLC columns for free glycans
The Thermo Scientific Accucore Amide HILIC column is best suited for the separation of hydrophilic biomolecules and for released glycan analysis.
| Catalog number | Product | Column ID (µm) | Column length (mm) | Flow rate range (nL/min) | Order |
|---|---|---|---|---|---|
| 16726-157569 | Amide HILIC column | 75 | 150 | 100 – 1000 | Order now › |
Emitters
These emitters provide a simple connection from your LC system to your EASY-Spray or Nanospray Flex ion source.
| Catalog number | Product | For use with | Order |
|---|---|---|---|
| ES993 | Nano EASY-Spray emitter, 10 µm ID | nanoViper columns | Order now › |
| ES994 | Capillary EASY-Spray emitter, 15 µm ID | nanoViper columns | Order now › |
| EMI-EASYSPRAYB | μPAC-compatible EASY-Spray emitter | µPAC columns | Order now › |
| EMI-FLEXIONB | μPAC Flex iON Connect ESI-MS interface | µPAC columns | Order now › |
Traps and accessories
For the best performance from your nanoViper columns, consider investing in the following accessories.
| Catalog number | Product | Particle/pillar diameter (µm) | Column ID (µm) | Media bed length (mm) | For use with | Order |
|---|---|---|---|---|---|---|
| 174500 | PepMap Neo trap cartridge, 1500 bar | 5 | 300 | 5 | nanoViper columns | Order now › |
| 164750 | PepMap nanotrap, 500 bar | 5 | 100 | 20 | nanoViper columns | Order now › |
| 164535 | PepMap nanotrap, 500 bar | 3 | 75 | 20 | nanoViper columns | Order now › |
| COL-TRPNANO16G1B2 | µPAC trapping column, 350 bar | 5 | 2000 | 10 | µPAC Neo columns | Order now › |
| Catalog number | Product | For use with | Order |
|---|---|---|---|
| 174501 | PEEK with nanoViper fittings, 30 µm X 100 mm, 1500 bar | nanoViper columns | Order now › |
| 174502 | Trap holder + nanoViper fittings kit, 1500 bar | nanoViper columns | Order now › |
| 6040.2304 | Union for Viper and nanoViper tubing | nanoViper columns | Order now › |
| 6041.5260 | nanoViper tubing, 20 μm x 550 mm | nanoViper columns | Order now › |
Thermo Scientific Vanquish Neo UHPLC System
The all-in-one nano-, capillary-, and micro-flow LC system for high sensitivity LCMS workflows.
Low-flow HPLC column videos
Low-flow HPLC columns powered with technology that's beyond brilliant
Learn more about the power of Thermo Scientific low-flow HPLC columns and discover column designs that ensure near zero-dead volume connections to give you confidence in your data quality.
µPAC HPLC column technology is a silicon revolution in chromatography
Our patented technology overcomes the physical limitations of packed bed LC columns. It reduces peak dispersion which leads to better separation. Furthermore, this unique technology increase data reproducibility and decrease costs as these columns are highly robust and reliable.
Technical University of Denmark (DTU): MAbPac RP 4 μm stand-alone and EASY-Spray LC columns and nano- and capillary-flow emitters
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