TaqMan Advanced miRNA Assays

Applied Biosystems TaqMan Advanced miRNA Assays enable highly sensitive and specific quantification of mature miRNAs using qPCR. Together with the TaqMan Advanced miRNA cDNA Synthesis Kit, this solution provides a streamlined upfront workflow that allows for exceptional detection of multiple miRNA targets from a single sample or low-level RNA samples such as serum and plasma.

  • Universal reverse transcription (RT)—one RT step for all TaqMan Advanced miRNA Assays.
  • Sensitive—unlike traditional pre-amplification, the miR-Amp primers in the ligation-based cDNA synthesis kit recognize the universal sequences added to every miRNA on the 5’ and 3’ ends, to help prevent amplification bias. Detect targets with as few as 60 copies of input microRNA (miRNA).
  • Specific—detect only mature miRNA and distinguish between closely related highly homologous miRNAs with our gold-standard TaqMan specificity.
  • Small sample input—detect and quantify mature miRNA from as little as 1 pg of total RNA or 2 μL of plasma or serum.
  • Versatile—compatible with tissue and biofluids including serum and plasma.
  TaqMan MicroRNA assays TaqMan Advanced miRNA assays
Description TaqMan MicroRNA assays employ a novel target-specific stem–loop primer during cDNA synthesis to produce a template for real-time PCR TaqMan Advanced miRNA assays employ a universal RT step for a streamlined workflow, and a universal miR-Amp step to enable highly sensitive detection by real-time PCR
RT chemistry miRNA-specific RT Universal RT
Throughput Best for 1–10 targets Best for >10 targets
Coverage 205 species available; coverage for miRBase v.21 All human, mouse, and rat miRNAs; coverage for miRBase v.21
Formats Available in individual tube, card, plate, and OpenArray format Available in individual tube, card, plate, and OpenArray formats. Inquire for custom plating options.

Now available: TaqMan Advanced miRNA assays in card, plate, and OpenArray format

Enable highly sensitive and specific quantification of mature miRNAs using qPCR.  Find your affordable solution using the search tool below.

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TaqMan miRNA Assay selection guide

Format

Focus areas

Applications

Recommended TaqMan assay/array:

Single tube

Quantitative miRNA  

TaqMan Advanced miRNA Assays
Requires only 1-10 nanograms of total RNA or equivalent and is ideal for a small number of assays (ideal for >10 targets with RT chemistry)
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96-well plate

Target miRNA analysis

Custom assays

Target miRNA analysis

TaqMan Advanced miRNA Assays
 Comprehensive collection of pre-plated, pre-designed panels to pinpoint your specific target; broad instrument compatibility
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384-well microfluidic card

Ideal for profiling studies on our ViiA 7 and 7900HT Fast real-time PCR systems

Comprehensive coverage of most relevant miRNA (miRBase v21) using precious limited samples or material (human/rodent)

Profiling selected miRNAs (using predesigned assays)

TaqMan Advanced miRNA array cards
Small reaction volumes and streamlined workflow enable profiling up to 2,300 miRNA targets from as little as 1 ng of total RNA
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Custom TaqMan miRNA array cards

 

OpenArray plate

Use with OpenArray system to meet cost-effective high-throughput profiling demands

Large-scale profiling (human/rodent)

TaqMan Advanced miRNA OpenArray panels
Run three samples per QuantStudio 12K Flex OpenArray plate, translating up to 818 assays per sample (up to 758 unique assays)
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Flexible content panels

TaqMan flexible content panels offer an easy way to select and design a combination of assays in the format that matches your experiment. We offer a wide range of flexible content panels.
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Custom assay services

Want us to produce a custom assay for you?
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Workflow

mirVana miRNA Isolation Kits

mirVana miRNA Isolation kits use a rapid procedure to isolate total RNA, including small RNAs such as miRNA, small interfering RNA (siRNA), and small nuclear RNA (snRNA), from tissues and cells.

TaqMan Advanced miRNA cDNA Synthesis Kit

The TaqMan Advanced miRNA cDNA Synthesis Kit is used for preparation of cDNA templates from the entire pool of mature miRNAs in a sample.

miR-Amp Universal Amplification

miR-Amp uses universal primers and master mix to uniformly increase the amount of cDNA for each target. miR-Amp reagents are included in the TaqMan Advanced miRNA cDNA Synthesis Kit.

Applied Biosystems real-time PCR instruments

With Applied Biosystems real-time PCR platforms, you get true value with excellent performance, reliability, and world-class support. Designed for compatibility with Applied Biosystems TaqMan Assays and the flexibility of unlimited real-time chemistry choices, we make it easy for you to get started with your experiments.

mirVana miRNA Isolation Kits

mirVana miRNA Isolation kits use a rapid procedure to isolate total RNA, including small RNAs such as miRNA, small interfering RNA (siRNA), and small nuclear RNA (snRNA), from tissues and cells.

TaqMan Advanced miRNA cDNA Synthesis Kit

The TaqMan Advanced miRNA cDNA Synthesis Kit is used for preparation of cDNA templates from the entire pool of mature miRNAs in a sample.

miR-Amp Universal Amplification

miR-Amp uses universal primers and master mix to uniformly increase the amount of cDNA for each target. miR-Amp reagents are included in the TaqMan Advanced miRNA cDNA Synthesis Kit.

Applied Biosystems real-time PCR instruments

With Applied Biosystems real-time PCR platforms, you get true value with excellent performance, reliability, and world-class support. Designed for compatibility with Applied Biosystems TaqMan Assays and the flexibility of unlimited real-time chemistry choices, we make it easy for you to get started with your experiments.

Species TaqMan Advanced miRNA assays are available for human miRNAs. We expect to increase the number of miRNA assays in alignment with the Sanger miRBase Registry.
Assay Components

TaqMan Advanced miRNA assays are delivered in a single tube containing the specific pre-formulated TaqMan Assay (TaqMan probe and PCR primer set)

Available Sizes
Product Catalog no. Number of reactions
TaqMan Advanced miRNA assays A25576 250 qPCR reactions (20 μL)
Shipping and Storage

Shipped at ambient temperature.
Store at –15ºC to—25ºC.

White Paper: Ambient Temperature Shipping Reduces Environmental Impact and Retains Quality and Stability

TaqMan Assay documentation files can be downloaded at thermofisher.com/taqmanfiles

White paper: TaqMan Advanced miRNA Assays - identifying normalizers
Product bulletin: TaqMan Advanced miRNA Assays
White paper: TaqMan Advanced miRNA Assays – superior performance
Your Innovative Research: Detection of miRNAs in nipple aspiration fluid
Application note: TaqMan Advanced miRNA Assays
User guide: TaqMan Advanced miRNA Assays
Quick reference: TaqMan Advanced miRNA Assays

Product Categories

Related Applications

Related Products

Data Analysis Tools

Species TaqMan Advanced miRNA assays are available for human miRNAs. We expect to increase the number of miRNA assays in alignment with the Sanger miRBase Registry.
Assay Components

TaqMan Advanced miRNA assays are delivered in a single tube containing the specific pre-formulated TaqMan Assay (TaqMan probe and PCR primer set)

Available Sizes
Product Catalog no. Number of reactions
TaqMan Advanced miRNA assays A25576 250 qPCR reactions (20 μL)
Shipping and Storage

Shipped at ambient temperature.
Store at –15ºC to—25ºC.

White Paper: Ambient Temperature Shipping Reduces Environmental Impact and Retains Quality and Stability

TaqMan Assay documentation files can be downloaded at thermofisher.com/taqmanfiles

White paper: TaqMan Advanced miRNA Assays - identifying normalizers
Product bulletin: TaqMan Advanced miRNA Assays
White paper: TaqMan Advanced miRNA Assays – superior performance
Your Innovative Research: Detection of miRNAs in nipple aspiration fluid
Application note: TaqMan Advanced miRNA Assays
User guide: TaqMan Advanced miRNA Assays
Quick reference: TaqMan Advanced miRNA Assays

Product Categories

Related Applications

Related Products

Data Analysis Tools

Innovative cloud-based qPCR analysis tools

Applied Biosystems qPCR analysis modules are intuitive cloud-based secondary data analysis solutions that combine multiple data sets in one convenient place, making it easier for you to view, store, and analyze your qPCR data.

Fast and powerful analysis— help save time and achieve more data with analytical speeds up to 50 times faster than desktop software, and processing power to analyze 500 TaqMan Advanced miRNA array cards or OpenArray plates

Customized visualization— efficiently derive insights from large data sets using visualization tools with one-click quality checks and one-click comparison, making it easy to identify outliers and filter data customized with attributes that are meaningful to your study

Integrated analysis solution— complete all your work in one location, analyze groupings of data such as time course experiments or cell line comparisons, and pick settings to easily compare data

Learn more ›

Resources

 White paper: TaqMan Advanced miRNA Assays
 Product Bulletin: TaqMan Advanced miRNA Assays
Applied Biosystems qPCR Analysis Modules
Software for microRNA and non-codingRNA analysis

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