Protein Sample Preparation

Protein extraction is an important initial step in biochemistry and molecular biology experiments that encompasses a wide range of techniques and reagents to isolate and purify specific proteins from biological samples. View a range of protein extraction reagents for cell lysis, organelle isolation, membrane protein extraction, and protease and phosphatase inhibition, each designed to support your diverse experimental needs.

Protein purification is essential for a host of biochemical applications. With thousands of proteins, each displaying unique characteristics, it is crucial to develop a strategy for purification that delivers the high yield, purity, and activity needed for downstream applications.

Dialysis membranes are used in the protein preparation workflow to remove unwanted small molecules, such as salts and solvents, from protein solutions through selective permeability. Dialysis products help maintain protein integrity and purity for downstream analyses and applications in research.

Perform biological sample concentration, desalting, and buffer exchange with Thermo Scientific Pierce protein concentrators, which are available as disposable ultrafiltration centrifugal devices and larger crossflow filtration cassettes in a range of sizes and MWCOs.

Desalting spin columns quickly and effectively remove salts and other small molecules from your sample using gel-filtration chromatography. Use with swinging-bucket or fixed-angle bench- or floor-model centrifuges. They are available in six volume capacities and two molecular weight cut-offs (MWCOs), 7K and 40K.

Eliminating unreacted or residual small molecules during protein sample preparation is crucial for successful downstream analysis. Our spin columns and 96-well filter plates feature a ready-to-use resin specifically designed for the quick removal of non-conjugated fluorescent dyes, biotin, unused reducing agents, and crosslinkers, helping ensure exceptional protein recovery.

Membrane protein extraction and isolation reagents

Pierce High Capacity Protein A Magnetic Beads, alkali stable

Nanocon Crossflow Concentrator

Protein expression systems, including algae, bacterial, mammalian, insect, and yeast expression vector systems that are easy to use, deliver protein at high yield, and are suitable for nearly every downstream application.

Protease and phosphatase inhibitors, cocktails, and tablets—excellent for the protection of proteins during extraction or lysate preparation from primary cells, cultured mammalian cells, animal tissues, plant tissues, yeast, or bacterial cells.

Follow these 5 steps to obtain optimal protein preparation conditions.

Follow these 5 steps to obtain optimal protein sample conditions.