These competent cells are optimized for protein expression from T7 promoters. All are induced with IPTG or L-arabinose and come in the convenient One Shot format, allowing for transformation and recovery in a single tube.
Which competent cell strain is right for you?
|High-level expression of recombinant proteins||Highest protein expression from low-copy plasmids||Highest protein expression from high-copy plasmids||Optimized or ideal for toxic proteins|
|Increased mRNA stability (mutated rne gene)|
|Expression of known toxic genes|
|Best expression vector format||pET-based expression vector (low copy number T7-based plasmids)||pET-based expression vector (low copy number T7-based plasmids)||pCR T7-based expression vector (high copy number, T7-based plasmids)||ANY|
T7-based expression vector
|Inducing agent||IPTG||IPTG||IPTG||Arabinose or Arabinose & IPTG|
|One Shot format||One Shot||One Shot||One Shot||One Shot|
|Kit contains||20 tubes x 50 µL||20 tubes x 50 µL||20 tubes x 50 µL||20 tubes x 50 µL|
|*BL21 Star (DE3) E. coli strain also available as higher throughput StripWell Format|
For Research Use Only. Not for use in diagnostic procedures.