Competent Cells for Routine Cloning | Thermo Fisher Scientific

Chemically competent and high transformation efficiency cells that do not require an electroporator.

Which competent cell strain is right for you?

	Economical; for routine subcloning	Mid-range transformation efficiency	High-efficiency, best all around	Highest efficiency, ideal for low starting DNA	High efficiency, fast growing
	Subcloning Efficiency DH5α Chemically Competent Cells	Library Efficiency DH5α Chemically Competent Cells	One Shot TOP10 Chemically Competent Cells*	One Shot OmniMAX T1^R Chemically Competent Cells*	One Shot Mach1 T1^R Chemically Competent Cells*
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Transformation Efficiency of pUC19	>1x10⁶ cfu/ug	>1x10⁸ cfu/ug	>1x10⁹ cfu/ug	>5x10⁹ cfu/ug (highest)	>1x10⁹ cfu/ug
One Shot format
T1 & T5 Phage resistant (ton A)
Time to pick colonies	Overnight	Overnight	Overnight	Overnight	Same day
Time to purify plasmid DNA after inoculation	Overnight	Overnight	Overnight	Overnight	As soon as 4 hrs
Blue/White Screening
Efficiently transform unmethylated DNA (hsd R)
Efficiently transform methylated DNA (mcr A)
Generate ssDNA (Contains F' Episome)
Kit contains	4 tubes x 500 µL	5 tubes x 200 µL	20 tubes x 50 µL	20 tubes x 50 µL	20 tubes x 50 µL
	Order	Order	Order	Order	Order
*E. coli strains also available as high-throughput MultiShot formats

For Research Use Only. Not for use in diagnostic procedures.