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One Stop. TOP10 competent cells. Go!
- Broad utility: cloning including mammalian and plant DNA, library construction, and methylated DNA
- Multiple formats: One Shot, MultiShot, Chemical, and Electroporation Competent
- High transformation efficiencies
Which TOP10 competent cells are right for you?
 One Shot TOP10 Chemically Competent E. coli |  One Shot TOP10 Electrocomp E. coli |  MultiShot StripWell TOP10 Chemically Competent E. coli |  MultiShot FlexPlate TOP10 Chemically Competent E. coli |  MultiShot TOP10 Chemically Competent E. coli | |
| Format | One Shot (pre-aliquoted, single use) | One Shot (pre-aliquoted, single use) | MultiShot StripWell (96-well StripWell format) | MultiShot FlexPlate (96-well plate divisible into 8-well segments) | MultiShot (96-well microtiter plate format) |
| Volume per vial or well (µL) | 50 | 50 | 50 | 50 | 15 |
| Number of vials or wells (smallest pack size) | 11 (includes 1 bonus vial that can be used for controls) | 11 (includes 1 bonus vial that can be used for controls) | 96 | 96 | 96 |
| Number of plates | N/A | N/A | 1 | 1 | 5 |
| Transformation efficiency
| >1 x 109 | >1 x 1010 | >1 x 108 | >1 x 108 | >1 x 108 |
| Type of transformation | Chemical | Electroporation | Chemical | Chemical | Chemical |
| Blue/white screening | |||||
| Transformation of methylated DNA from genomic preparations | |||||
| Order | Order | Order | Order | Order |
References
| Applications | Publications |
|---|---|
| One Shot TOP10 chemically competent cells are used for the generation of a scFv library and enrichment of scFv clones. | Pastan I, Ho M. (2009) Display and selection of scFv antibodies on HEK-293T cells.Methods Mol Biol. 562: 99–113. |
| Construction of genome editing nuclease protein expression vectors use TOP10 chemically competent cells. | Barbas III C, Liu J, et al. (2015) Efficient delivery of nuclease proteins for genome editing in human stem cells and primary cells. Nature Protocols 10(11)1842 –1859. |
| Zika studies enabled through generation of cDNA and cloning using TOP10 cells. | Shan C, Shi P, et al. (2016) An Infectious cDNA Clone of Zika Virus to Study Viral Virulence, Mosquito Transmission, and Antiviral Inhibitors. Cell Host & Microbe 19:891–900. |
Resources
TOP10 product manuals
Protocols
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Competent cells resources
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