One Stop. TOP10 competent cells. Go!

  • Broad utility: cloning including mammalian and plant DNA, library construction, and methylated DNA 
  • Multiple formats: One Shot, MultiShot, Chemical, and Electroporation Competent 
  • High transformation efficiencies


Which TOP10 competent cells are right for you?

  One Shot TOP10 Chemically Competent E. coli
One Shot TOP10 Chemically Competent E. coli
One Shot TOP10 Electrocomp E.Coli
One Shot TOP10 Electrocomp E. coli
MultiShot StripWell TOP10 Chemically Competent E.Coli
MultiShot StripWell TOP10 Chemically Competent E. coli
MultiShot FlexPlate TOP10 Chemically Competent E.Coli
MultiShot FlexPlate TOP10 Chemically Competent E. coli
MultiShot TOP10 Chemically Competent E.Coli
MultiShot TOP10 Chemically Competent E. coli
Format One Shot
(pre-aliquoted, single use)
One Shot
(pre-aliquoted, single use)
MultiShot StripWell
(96-well StripWell format)
MultiShot FlexPlate
(96-well plate divisible into 8-well segments)
MultiShot
(96-well microtiter plate format)
Volume per vial or well (µL) 50 50 50 50 15
Number of vials or wells (smallest pack size) 11
(includes 1 bonus vial that can be used for controls)
11
(includes 1 bonus vial that can be used for controls)
96 96 96
Number of plates N/A N/A 1 1 5
Transformation efficiency  (cfu/µg) >1 x 109 >1 x 1010 >1 x 108 >1 x 108 >1 x 108
Type of transformation Chemical Electroporation Chemical Chemical Chemical
Blue/white screening          
Transformation of methylated DNA from genomic preparations          
  Order Order Order Order Order

References

Applications Publications
One Shot TOP10 chemically competent cells are used for the generation of a scFv library and enrichment of scFv clones. Pastan I, Ho M. (2009) Display and selection of scFv antibodies on HEK-293T cells. Methods Mol Biol. 562: 99–113.
Construction of genome editing nuclease protein expression vectors use TOP10 chemically competent cells. Barbas III C, Liu J, et al. (2015) Efficient delivery of nuclease proteins for genome editing in human stem cells and primary cells. Nature Protocols 10(11)1842 –1859.
Zika studies enabled through generation of cDNA and cloning using TOP10 cells. Shan C, Shi P, et al. (2016) An Infectious cDNA Clone of Zika Virus to Study Viral Virulence, Mosquito Transmission, and Antiviral Inhibitors. Cell Host & Microbe 19:891–900.

Resources

Competent cells resources

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