SureLock Tandem Midi Gel Tank

Increase your output with the Invitrogen SureLock Tandem Midi Gel Tank, designed for easy and consistent vertical protein gel electrophoresis of 1 or 2 Invitrogen midi gels. When paired with SureLock Midi Transfer Module, this tank performs efficient, room-temperature, wet protein transfers for downstream western blot analysis.

Power Supplies  Protein Gels

Features

  • 2-in-1 midi gel electrophoresis and transfer tank—run and transfer high-performance Invitrogen midi gels using the same tank.
  • Two separate gel chambers—run 1 or 2 gels or transfers using only the necessary amount of buffer for each gel, minimizing buffer cost and waste.
  • User-friendly—designed for easy set-up and use.
  • Optimal performance with fast transfer protocols—efficient, room-temperature transfers in 30 minutes.
  • Room temperature transfer—eliminate the need to pre-chill buffers and the hassle and messiness of ice baths.
  • Compatible—fits all Invitrogen precast midi gels and Invitrogen midi gel cassettes.
  • Durable polycarbonate construction—the SureLock Tandem Midi Gel Tank is built to last.
     

SureLock Tandem Midi Gel Tank specifications

Capacity Up to 2 midi gels
Gel compatibility Gel size: 8 x 13cm
Gel cassette: 10.3 x 15cm
Thickness: 1.0mm
Compatible gels All Invitrogen precast midi gels, and Invitrogen empty midi gel cassettes
Buffer chamber requirement Upper chamber: 170 mL (per gel)
Lower chamber: 350 mL (per gel)
Compatible power supplies PowerEase Power Supplies, Zoom Dual Power, or Owl systems.
Transfer module SureLock Tandem Midi Blot Module
Unit dimensions 25 × 18 × 17 cm (height with lid)
Material Polycarbonate
Electrode wire Platinum
Electrode limits 300 VDC or 250 Watts
Chemical resistance Impervious to most alcohols but not compatible with chlorinated hydrocarbons (e.g., chloroform), aromatic hydrocarbons (e.g., toluene, benzene), acetone, or isopropyl alcohol.
Catalog number STM1001

Description

The SureLock Tandem Midi Gel Tank is uniquely designed to enable convenient, reliable gel electrophoresis and protein transfer of high-performance Invitrogen midi gels. You can perform vertical electrophoresis of one or two midi gels, or transfer of one or two midi gels using the SureLock Tandem Midi Blot Module. Separate chambers make it easy to run just one gel or transfer, allowing you to save on buffer and limit methanol waste. Wet transfers can be performed in 30 minutes at room temperature, and there is no need to prechill buffers overnight or freeze cooling accessories before use.

The SureLock Tandem Midi Gel Tank is compatible with all Invitrogen precast midi gels and Invitrogen handcast midi gel cassettes, providing you with an effective and efficient 2-in-1 solution for producing publication-quality high throughput electrophoresis and western blotting results.

The SureLock Tandem Midi Gel Tank can be purchased separately or in a variety of product bundles. It can be purchased in a welcome pack with the SureLock Tandem Midi Blot Module and PVDF or nitrocellulose membranes, and SureLock Tandem Transfer Tray. The SureLock Tandem Midi Gel Tank is also included with Invitrogen midi gels, buffers, and ladders, at no extra charge in several Protein Gels Welcome Packs. See the Ordering tab for more information.

SureLock Tandem Midi welcome pack

SureLock Tandem Midi welcome pack

SureLock Tandem Transfer Tray

SureLock Tandem Transfer Tray

The SureLock Tandem Midi Gel Tank provides for rapid running of midi gels using minimal buffer in a leak-free system. With a set-up time of ~30 seconds, the tank efficiently runs midi gels while also providing consistent performance.

Run conditions

Run conditions for various types of midi gels are provided below. We recommend performing electrophoresis at constant voltage. Note that run-time values are approximate and will vary depending on gel percentage.

Gel Type (running buffer) Constant Voltage (V) Run Time (min)
Bis-Tris (MES SDS Running Buffer) 200 30
Bis-Tris (MOPS SDS Running Buffer) 200 40
Tris-glycine Plus (Tris-Glycine SDS Running Buffer) 200 60
Tris-glycine Plus (Tris-Glycine Native Running Buffer) 125 120
Tris-acetate (Tris-acetate SDS Running Buffer) 150 60
Tris-acetate (Tris-Glycine Native Running Buffer) 150 135

Publication-quality protein electrophoresis data is easily generated using the SureLock Tandem Midi Gel Tank and Invitrogen precast midi gels as illustrated in Figure 1, below. In these examples, the same samples were separated on a NuPAGE Bis-Tris 4–12% midi gradient gel and on a Novex Tris-Glycine Plus 4–12% midi gradient gel, using the SureLock Tandem Midi Gel Tank. Both gels produced crisp bands and straight protein lanes. In the NuPAGE gel example, the combination of the gradient gel and the MOPS buffer resolved the protein bands across a broader range of the gel.

Figure 1. Publication-quality protein electrophoresis gel results using NuPAGE Bis-Tris 4-12% and Novex Tris-Glycine Plus 4-12% gradient midi gels and SureLock Tandem Midi Gel Tank. Both gels were loaded as follows: Lanes 1, 20: 5 µL PageRuler Broad Range Unstained Protein Ladder (Cat. No. 26630); lanes 2-7: 10 µg, 8 µg, 6 µg, 4 µg, 2 µg, 1 µg HeLa lysate, respectively; lanes 8, 13: 5 µL Novex Mark12 Unstained Standard (Cat. No. LC5677); lanes 9-12: 240 ng, 180 ng, 120 ng, 60 ng of protein mix containing β-galactosidase, lactate dehydrogenase, and lysozyme; lanes 14-19: 10 µg, 8 µg, 6 µg, 4 µg, 2 µg, 1 µg E. coli lysate, respectively. Electrophoresis was conducted with NuPAGE MOPS running buffer, and Novex Tris-glycine running buffer for each corresponding gel. Gels were stained with SimplyBlue Safe Stain.

Publication-quality, multiplexed western blotting data can be generated using the SureLock Tandem Midi Gel Tank, Blot Modules, and Invitrogen precast midi gels as illustrated in Figure 2, below. In these examples, the same samples were separated on NuPAGE Bis-Tris 4–12% midi gradient gels using the SureLock Tandem Midi Gel Tank and transferred using SureLock Tandem Midi Blot Modules. One gel was transferred to a 0.2 µm nitrocellulose membrane and the second to a 0.45 µm PVDF membrane, and both were processed with the same western blotting protocol, resulting in multiplexed blots with crisp, bright bands with straight lanes.

Figure 2. Multiplex western blotting following separation in the SureLock Tandem Midi Gel Tank and wet tank transfer using SureLock Tandem Midi Blot Modules generates excellent results on both nitrocellulose and PVDF membranes. A 2-fold dilution series of A431 cell lysate starting at 1.0 µg/µL was used along with iBright Prestained Protein Ladder. 10 µL of each A431 lysate concentration was loaded in each of 2 NuPAGE 4–12% Bis-Tris, 20-well gels and separated by electrophoresis using the SureLock Tandem Midi Gel Tank with the Invitrogen PowerEase Touch 350 W Power Supply and pre-programmed protocol NuPAGE BT (MOPS) (200 V; 40 min). Proteins were transferred to either a 0.2 µm nitrocellulose membrane or a 0.45 µm PVDF membrane using the SureLock Tandem Midi Blot Module in the SureLock Tandem Midi Gel Tank at 25 V for 30 min. Membranes were processed using the Invitrogen Bandmate™ Automated Western Blot Processor. Membranes were blocked in 1X Clear Milk Blocking Buffer. For fluorescence multiplex detection, the membranes were probed with a mixture of primary antibodies diluted in 1X blocking solution containing rabbit-anti EGFR (1:1,250) (Cat. No. PA1-1110), rabbit-anti Hsp90 (1:4,000) (Cat. No. PA3-013), chicken anti-Calreticulin (1:4,000) (Cat. No. PA1-903), mouse anti-actin (1:1,250) (Cat. No. MA1-140), and mouse anti-p23 (1:1,250) (Cat. No. MA3-414) followed by an incubation with secondary antibodies diluted at 1:5,000 in 1X blocking solution: Goat anti-Mouse Alexa Fluor Plus 488 (Cat. No. A32723), Goat anti-Chicken Alexa Fluor 546 (Cat. No. A11040), and Goat anti-Rabbit Alexa Fluor Plus (Cat. No. A32735). Membranes were imaged on the iBright FL1500 with 3x3 binning, and 1.0X zoom.

SureLock Tandem Midi Gel Tank specifications

Capacity Up to 2 midi gels
Gel compatibility Gel size: 8 x 13cm
Gel cassette: 10.3 x 15cm
Thickness: 1.0mm
Compatible gels All Invitrogen precast midi gels, and Invitrogen empty midi gel cassettes
Buffer chamber requirement Upper chamber: 170 mL (per gel)
Lower chamber: 350 mL (per gel)
Compatible power supplies PowerEase Power Supplies, Zoom Dual Power, or Owl systems.
Transfer module SureLock Tandem Midi Blot Module
Unit dimensions 25 × 18 × 17 cm (height with lid)
Material Polycarbonate
Electrode wire Platinum
Electrode limits 300 VDC or 250 Watts
Chemical resistance Impervious to most alcohols but not compatible with chlorinated hydrocarbons (e.g., chloroform), aromatic hydrocarbons (e.g., toluene, benzene), acetone, or isopropyl alcohol.
Catalog number STM1001

Description

The SureLock Tandem Midi Gel Tank is uniquely designed to enable convenient, reliable gel electrophoresis and protein transfer of high-performance Invitrogen midi gels. You can perform vertical electrophoresis of one or two midi gels, or transfer of one or two midi gels using the SureLock Tandem Midi Blot Module. Separate chambers make it easy to run just one gel or transfer, allowing you to save on buffer and limit methanol waste. Wet transfers can be performed in 30 minutes at room temperature, and there is no need to prechill buffers overnight or freeze cooling accessories before use.

The SureLock Tandem Midi Gel Tank is compatible with all Invitrogen precast midi gels and Invitrogen handcast midi gel cassettes, providing you with an effective and efficient 2-in-1 solution for producing publication-quality high throughput electrophoresis and western blotting results.

The SureLock Tandem Midi Gel Tank can be purchased separately or in a variety of product bundles. It can be purchased in a welcome pack with the SureLock Tandem Midi Blot Module and PVDF or nitrocellulose membranes, and SureLock Tandem Transfer Tray. The SureLock Tandem Midi Gel Tank is also included with Invitrogen midi gels, buffers, and ladders, at no extra charge in several Protein Gels Welcome Packs. See the Ordering tab for more information.

SureLock Tandem Midi welcome pack

SureLock Tandem Midi welcome pack

SureLock Tandem Transfer Tray

SureLock Tandem Transfer Tray

The SureLock Tandem Midi Gel Tank provides for rapid running of midi gels using minimal buffer in a leak-free system. With a set-up time of ~30 seconds, the tank efficiently runs midi gels while also providing consistent performance.

Run conditions

Run conditions for various types of midi gels are provided below. We recommend performing electrophoresis at constant voltage. Note that run-time values are approximate and will vary depending on gel percentage.

Gel Type (running buffer) Constant Voltage (V) Run Time (min)
Bis-Tris (MES SDS Running Buffer) 200 30
Bis-Tris (MOPS SDS Running Buffer) 200 40
Tris-glycine Plus (Tris-Glycine SDS Running Buffer) 200 60
Tris-glycine Plus (Tris-Glycine Native Running Buffer) 125 120
Tris-acetate (Tris-acetate SDS Running Buffer) 150 60
Tris-acetate (Tris-Glycine Native Running Buffer) 150 135

Publication-quality protein electrophoresis data is easily generated using the SureLock Tandem Midi Gel Tank and Invitrogen precast midi gels as illustrated in Figure 1, below. In these examples, the same samples were separated on a NuPAGE Bis-Tris 4–12% midi gradient gel and on a Novex Tris-Glycine Plus 4–12% midi gradient gel, using the SureLock Tandem Midi Gel Tank. Both gels produced crisp bands and straight protein lanes. In the NuPAGE gel example, the combination of the gradient gel and the MOPS buffer resolved the protein bands across a broader range of the gel.

Figure 1. Publication-quality protein electrophoresis gel results using NuPAGE Bis-Tris 4-12% and Novex Tris-Glycine Plus 4-12% gradient midi gels and SureLock Tandem Midi Gel Tank. Both gels were loaded as follows: Lanes 1, 20: 5 µL PageRuler Broad Range Unstained Protein Ladder (Cat. No. 26630); lanes 2-7: 10 µg, 8 µg, 6 µg, 4 µg, 2 µg, 1 µg HeLa lysate, respectively; lanes 8, 13: 5 µL Novex Mark12 Unstained Standard (Cat. No. LC5677); lanes 9-12: 240 ng, 180 ng, 120 ng, 60 ng of protein mix containing β-galactosidase, lactate dehydrogenase, and lysozyme; lanes 14-19: 10 µg, 8 µg, 6 µg, 4 µg, 2 µg, 1 µg E. coli lysate, respectively. Electrophoresis was conducted with NuPAGE MOPS running buffer, and Novex Tris-glycine running buffer for each corresponding gel. Gels were stained with SimplyBlue Safe Stain.

Publication-quality, multiplexed western blotting data can be generated using the SureLock Tandem Midi Gel Tank, Blot Modules, and Invitrogen precast midi gels as illustrated in Figure 2, below. In these examples, the same samples were separated on NuPAGE Bis-Tris 4–12% midi gradient gels using the SureLock Tandem Midi Gel Tank and transferred using SureLock Tandem Midi Blot Modules. One gel was transferred to a 0.2 µm nitrocellulose membrane and the second to a 0.45 µm PVDF membrane, and both were processed with the same western blotting protocol, resulting in multiplexed blots with crisp, bright bands with straight lanes.

Figure 2. Multiplex western blotting following separation in the SureLock Tandem Midi Gel Tank and wet tank transfer using SureLock Tandem Midi Blot Modules generates excellent results on both nitrocellulose and PVDF membranes. A 2-fold dilution series of A431 cell lysate starting at 1.0 µg/µL was used along with iBright Prestained Protein Ladder. 10 µL of each A431 lysate concentration was loaded in each of 2 NuPAGE 4–12% Bis-Tris, 20-well gels and separated by electrophoresis using the SureLock Tandem Midi Gel Tank with the Invitrogen PowerEase Touch 350 W Power Supply and pre-programmed protocol NuPAGE BT (MOPS) (200 V; 40 min). Proteins were transferred to either a 0.2 µm nitrocellulose membrane or a 0.45 µm PVDF membrane using the SureLock Tandem Midi Blot Module in the SureLock Tandem Midi Gel Tank at 25 V for 30 min. Membranes were processed using the Invitrogen Bandmate™ Automated Western Blot Processor. Membranes were blocked in 1X Clear Milk Blocking Buffer. For fluorescence multiplex detection, the membranes were probed with a mixture of primary antibodies diluted in 1X blocking solution containing rabbit-anti EGFR (1:1,250) (Cat. No. PA1-1110), rabbit-anti Hsp90 (1:4,000) (Cat. No. PA3-013), chicken anti-Calreticulin (1:4,000) (Cat. No. PA1-903), mouse anti-actin (1:1,250) (Cat. No. MA1-140), and mouse anti-p23 (1:1,250) (Cat. No. MA3-414) followed by an incubation with secondary antibodies diluted at 1:5,000 in 1X blocking solution: Goat anti-Mouse Alexa Fluor Plus 488 (Cat. No. A32723), Goat anti-Chicken Alexa Fluor 546 (Cat. No. A11040), and Goat anti-Rabbit Alexa Fluor Plus (Cat. No. A32735). Membranes were imaged on the iBright FL1500 with 3x3 binning, and 1.0X zoom.

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