Our comprehensive 2′ modified RNA phosphoramidite portfolio is uniquely designed for nucleic acid based therapeutics research. Easily scale up your synthesis of siRNA (small interfering RNA) and ASO (antisense oligonucleotide) RNA therapeutics from early-stage development with the comprehensive supply of Thermo Scientific's phosphoramidites. 

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Thermo Scientific 2′ modified RNA phosphoramidites

Modifications at the 2′ position of the ribose ring are commonly used to help increase oligonucleotide stability and improve resistance to nuclease activity in vivo. Our 2'-modified RNA phosphoramidites are manufactured to precise standards to help ensure quality, consistency, and reliability for nucleic acid-based therapeutics research. Choose from different grades (TheraPure and standard) as well as fast deprotect phosphoramidites.


2'-OMe phosphoramidites

TheraPure and standard grades 2′-OMe phosphoramidites are available for oligo synthesis research. For faster deprotection times, consider reviewing PAC/acetyl chemistries. 

Available 2′-OMe modified RNA phosphoramidites by synthesizer compatibility

Modification

2'-OMe phosphoramidites

Common applicationssiRNA researchDiagnostics research

Quality grades

Standard

Featuring quality attribute

  • TheraPure: Maximal QC and analytical testing supported by documentation
  • Standard: Highly reliable for routine oligo synthesis
Allow the removal of base-protecting groups under mild or accelerated conditions 
 Bz-AUiBu GAc CPAC-AiPrPAC-G
Applied Biosystems/AKTA Synthesizer27-2042-0427-2044-0427-2046-0427-2043-0427-1822-0227-1826-02
Expedite
Synthesizer		27-2042-1027-2044-1027-2046-1027-2043-1027-1822-1027-1826-10
MerMade
Synthesizer

Custom orders are available.
Request quote

27-1822-1127-1826-11

Contact us for other desired pack sizes including for acetyl protected 2'-OMe G.


Manufacturing for therapeutics research with high purity requirements

Quality attributes of 2′-OMe phosphoramidites are presented in the table below.

  Click images to enlarge

 2′-OMe-Bz-A-CEP2′-OMe-U-CEP2′-OMe-iBu-G-CEP2′-OMe-Ac-C-CEP
TheraPure grade Cat. No.27-204227-204427-204627-2043
Standard grade Cat. No.27-184227-182527-184627-1823
FormulaC48H54N7O8PC40H56N7O9PC45H56N7O9PC42H52N5O9P
Molecular weight887.97 g/mol760.8 g/mol869.9 g/mol801.86 g/mol
CAS #110782-31-5110764-79-9150780-67-9199593-09-4
AppearanceWhite to pale yellow powder
HPLC purity (TheraPure phosphoramidites*)≥98.0% | ≥99% *
31P NMR purity (TheraPure phosphoramidites**)≥ 98.0% | ≥99% **
Shipping / Storage  conditionAmbient / –20 °C
Water content ≤ 0.2% (KF Method)
Material originNon-animal source (no TSE/BSE risk)

Residual solvents: </= 3.0% (for TheraPure Grade only)
* Additional specifications for non-critical, critical, and sum of critical impurities are available with TheraPure grade.
** Additional specifications for single impurities and sum of impurities are available with TheraPure grade.


Compatible products by synthesizer model

Available 2′-OMe phosphoramidites in TheraPure or standard grades and are compatible with the following oligonucleotide synthesizers: 

  • Applied Biosystems / AKTA Synthesizer
  • Expedite Synthesizer
  • MerMade Synthesizer—Custom order only; contact us for a quote
Compatible products with the Applied Biosystems and AKTA synthesizer

Compatible products are in amber bottles with serum vial neck style as ready-to-ship catalog SKU for your siRNA and ASO synthesis.

Expedite synthesizer

Available catalog SKUs are supplied in 30 mL amber bottle with a 20-400 finish neck style. Compatible with the Expedite synthesizer.

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Manufacturing for diagnostic applications

Utilize PAC-A and iPrPAC-G / Ac-G with Ac-C and U to minimize deprotection times.

  Click images to enlarge

 2′-OMe-PAC-A-CEP2′-OMe-iPrPAC-G-CEPTheraPure 2'-OMe-Ac-G-CEP
Cat. No.27-182227-1826SY5400†
FormulaC49H56N7O9PC52H62N7O10PC43H52N7O9P
Molecular weight918.0 g/mol976.1 g/mol841.9 g/mol
CAS #128219-82-9NA909033-40-5
HPLC purity≥ 98.0%≥ 98.0%≥ 99%
31P NMR purity≥ 98.0%≥ 98.0%≥ 99%
HazardousNo
Shipping / Storage conditionAmbient / –20°C
Material originNon-animal source (no TSE/BSE risk)
C52H62N7O10P

† For SY5400: Residual solvents: ≤ 3.0%. Water content: ≤ 0.2%. To order TheraPure 2'-OMe-Ac-G-CEP, please request a quote.

Compatibility by synthesizer model

2′-OMe modified RNA phosphoramidites are compatible with the following oligonucleotide synthesizers: 

  • Applied Biosystems and AKTA Synthesizer
  • Expedite Synthesizer
  • MerMade Synthesizer
Applied Biosystems and AKTA synthesizer

Compatible catalog products are ready-to-ship in amber bottles with serum vial neck style.

Expedite synthesizer

Available catalog SKUs are supplied in 30 mL amber bottle with a 20-400 finish neck style. 

MerMade systems synthesizer

Compatible products are available to ship in a 30 mL amber bottle with a 28-400 finish neck style.

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2'-Fluoro Phosphoramidites

Synthesizing RNA oligonucleotides using 2′-Fluoro Bz A, iBu G, Ac C, and U phosphoramidites increases the thermal stability of RNA duplexes and increases the nuclease resistance of RNA. These phosphoramidites can be incorporated into custom siRNA oligonucleotides for use in gene-targeting and suppression research studies.

Available 2'-Fluoro modified phosphoramidites by compatible synthesizers

Modifications2'-Fluoro phosphoramidites
Common applicationssiRNA research
Quality gradesTheraPure
Featuring quality attributeMaximal QC and analytical testing supported by documentation
 Ac-CUBz-AiBu-G

Applied Biosystems/AKTA Synthesizer

27-1604-0227-1602-0227-1601-0227-1607-02
Expedite
Synthesizer		27-1604-0927-1602-0927-1601-0927-1607-09
MerMade
Synthesizer		27-1604-12

27-1602-12

 

27-1601-1227-1607-12


Technical specifications for 2'-Fluoro modification

  Click images to enlarge

 2′-F-Ac-C-CEP2′-F-U-CEP2′-F-Bz-A-CEP2′-F-iBu-G-CEP
Quality gradeTherapure
Cat. No.27-160427-160227-160127-1607
FormulaC41H49N5FO8PC39H46FN4O8PC47H51FN7O7PC44H53FN7O8P
Molecular weight798.8 g/mol748.8 g/mol875.92 g/mol857.4 g/mol
CAS #159414-99-0146954-75-8N/A144089-97-4
Residual solvent≤3.0% total % by weight solvent
AppearanceWhite to light yellow powder
HPLC purity≥99%
31P NMR purity≥99%
Solution clarityFree from undissolved particulate at 0.2 M solution in ACN
Shipping / Storage conditionAmbient / –20°C
Water content≤0.3%
Material originNon-animal source (no TSE/BSE risk)


Compatibility by synthesizer model

2′-Fluoro modified RNA phosphoramidites are compatible with the following oligonucleotide synthesizers: 

  • Applied Biosystems / AKTA Synthesizer
  • Expedite Synthesizer
  • MerMade Synthesizer

Applied Biosystems and AKTA synthesizer

Order compatible products in amber bottles with serum vial neck style.

Expedite synthesizer

Available catalog SKUs are supplied in 30 mL amber bottle with a 20-400 finish neck style.

MerMade systems synthesizer

Catalog SKUs available that are compatible with the MerMade synthesizer in a 30 mL amber bottle with a 28-400 finish neck style.

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2'-MOE phosphoramidites

Oligonucleotides synthesized using 2′-MOE modifications have been shown to be more nuclease resistant, with lower toxicity, and slightly increased hybridization affinities, making them well suited for therapeutics research.

  • Tightly controlled, critical impurities
  • Reliable, high quality oligonucleotide synthesis

Available 2'-MOE modified phosphoramidites by compatible synthesizers

Modifications2'-MOE phosphoramidites
Common applicationsASO research
Quality gradesTheraPure
Featuring quality attributeMaximal QC testing to help ensure impurities and residual solvents are within the stringent requirement for oligo therapeutics research
 Bz-ABz-5mCiBu-GT
Applied Biosystems/AKTA Synthesizer27-1019-0227-1020-0227-1022-0227-1021-02
Expedite
Synthesizer

Custom orders are available.
Request quote

MerMade
Synthesizer		27-1019-1127-1020-1127-1022-1127-1021-11


Technical specifications of 2'-MOE modification

  Click images to enlarge

 2′-MOE-A CEP2′-MOE-5mC CEP2′-MOE-G CEP2′-MOE-T CEP
Quality gradeTheraPureTheraPureTheraPureTheraPure
Cat. No.27-101927-102027-102227-1021
FormulaC50H58N7O9PC50H60N5O10PC47H60N7O10PC43H55N4O10P
Molecular weight932.03 g/mol922.03 g/mol914.01 g/mol818.90 g/mol
CAS #251647-53-7163759-94-2251647-55-9163878-63-5
Solution clarityFree from undissolved particulate at 0.2 M solution in ACN
HPLC purity*

 ≥99%

31P NMR purity*≥99%
Shipping / Storage  conditionAmbient / –20°C
Water content≤0.3% (by KF Method)
Total residual solvents≤4.0%
Material originNon-animal source (no TSE/BSE risk)

Compatibility by synthesizer model

TheraPure RNA phosphoramidites with 2′-MOE modifications are compatible with the following oligonucleotide synthesizers: 

  • Applied Biosystems / AKTA Synthesizer
  • Expedite Synthesizer: Custom order only; contact us for a quote
  • MerMade Synthesizer
Applied Biosystems and AKTA synthesizer

Compatible catalog products of 1 g or 5 g are ready-to-ship in amber bottles with serum vial neck style. 

MerMade systems synthesizer

Catalog SKUs compatible to MerMade synthesizer are ready-to-ship in 30 mL amber bottle with a 28-400 finish neck style. Sizing options of 1 g or 2 g are available to choose from.

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Looking for additional size options, customization, or packaging solutions?

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Resources

Phosphoramidite Learning Resource

Access webinars, videos, articles, and more to optimize your success in oligonucleotide synthesis for diagnostic and therapeutic manufacturing process development.

mRNA Production Learning Library

Browse the educational assets related to raw materials selection, process development using in vitro transcription, scale-up synthesis, and the latest trends in mRNA production.

Stylesheet for Classic Wide Template adjustments

For Research Use Only. Not for use in diagnostic procedures.