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Useful Numbers for Cell Culture
Useful cell culture numbers for various sizes of dishes and flasks
There are various sizes of dishes and flasks used for cell culture. Some useful cell culture numbers such as surface area and volumes of dissociation solutions are given below for various size culture vessels.
| Cat. No. | Surface area (cm2) | Seeding density* | Cells at confluency† | Versene (mL of 0.05% EDTA), approx. volume | Trypsin (mL of 0.05% trypsin, 0.53 mM EDTA), approx. volume | Growth medium (mL), approx. volume | |
|---|---|---|---|---|---|---|---|
| Dishes | |||||||
| 35 mm | 150460 150318 | 8.8 | 0.3 x 106 | 1.2 x 106 | 1 | 1 | 2 |
| 60 mm | 150462 150288 | 21.5 | 0.8 x 106 | 3.2 x 106 | 3 | 3 | 5 |
| 100 mm | 150464 150350 | 56.7 | 2.2 x 106 | 8.8 x 106 | 5 | 5 | 12 |
| 150 mm | 150468 168381 | 145 | 5.0 x 106 | 20.0 x 106 | 10 | 10 | 30 |
| Culture plates | |||||||
| 6-well | 140675 | 9.6 | 0.3 x 106 | 1.2 x 106 | 1 | 1 | 1 to 3 |
| 12-well | 150628 | 3.5 | 0.1 x 106 | 0.5 x 106 | 0.4 to 1 | 0.4 to 1 | 1 to 2 |
| 24-well | 142475 | 1.9 | 0.05 x 106 | 0.24 x 106 | 0.2 to 0.3 | 0.2 to 0.3 | 0.5 to 1.0 |
| 48-well | 150687 | 1.1 | 0.03 x 106 | 0.12 x 106 | 0.1 to 0.2 | 0.1 to 0.2 | 0.2 to 0.4 |
| 96-well | 167008 | 0.32 | 0.01 x 106 | 0.04 x 106 | 0.05 to 0.1 | 0.05 to 0.1 | 0.1 to 0.2 |
| 384-well | 164688 | 0.084 | 0.0018 x 106 | 0.0072 x 106 | 0.015 | 0.015 | 0.08 |
| 1536-well | 253614 | 0.03 | 0.0009 x 106 | 0.0036 x 106 | 0.003 | 0.003 | 0.01 |
| Flasks | |||||||
| T-25 | 156367 156340 | 25 | 0.7 x 106 | 2.8 x 106 | 3 | 3 | 3 to 5 |
| T-75 | 156499 156472 | 75 | 2.1 x 106 | 8.4 x 106 | 5 | 5 | 8 to 15 |
| T-175 | 159910 159920 | 175 | 4.9 x 106 | 23.3 x 106 | 17 | 17 | 35 to 53 |
| T-225 | 159934 159933 | 225 | 6.3 x 106 | 30 x 106 | 22 | 22 | 45 to 68 |
| *Seeding density is given for each culture vessel type as follows: Dishes and Flasks: Cells per vessel; Culture plates: Cells per well. †The number of cells on a confluent plate, dish, or flask will vary with cell type. For this table, HeLa cells were used. | |||||||
Useful tools for cell culture
Thermo Fisher Scientific offers a wide array of helpful tools and resources to help you find the best products for your work:
Gibco Media Selection Tool
Use the interactive Gibco Media Selection Tool to find a media solution suited to your specific application. Select from catalog media or personalize your formulation to fit your experimental needs and budget.
Cell Culture Select Tool
Use the Cell Culture Select Tool to find resources to help you culture with confidence. Enter your cell line to find recommended, media, sera, and cultureware to support cell growth and maintenance.
Transfection Select Tool
The TransfectionSelect tool can help you find transfection products, protocols, and citations for your research. Enter details about your research and application and receive transfection product recommendations.
Nunc Plate Selection Guide
From single plates to 1536 well plates, there is a Thermo Scientific Nunc plate for your needs. Use the Nunc Plate Selection guide to find plates quickly using the drop menu. Bookmark your selections and order directly!
Switch-to-Nunc Cross Reference
Use the Switch-to-Nunc tool to find replacements for cell culture plastics from brands like Corning, Costar, Falcon, and more. Enter a supplier's SKU to find Nunc equivalents, or filter by brand, category, and size.
Switch-to-Gibco Cross Reference
Try the Switch-to-Gibco Product cross-reference tool. Enter your current cell culture product catalog number to get a recommended Gibco product replacement for consistent and reliable results.
Sera Lot Matching Tool
Use the Gibco iMATCH Sera Lot Matching Tool to find an FBS match in seconds. Provide your previous Gibco FBS lot number or answer a few quick questions to find your ideal match. Stop testing and start matching today!
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Recommended products
Grow and maintain a variety of mammalian cell lines with Gibco Cell Culture Media.
Nunc Cell Culture Plastics support every step of your research with a broad range of culture vessels.
Gibco FBS offers consistent growth for your cells through essential nutrients and growth factors.
Resources
Cell Culture & Transfection Learning Center
Access cell culture and transfection educational resources to enable excellent experiment planning and execution.
Gibco Cell Culture Basics
Learn best practices and fundamentals of cell culture. Topics include aseptic technique and methods for the growth and maintenance of cells in culture.
Cell Culture Videos
Explore this collection of cell culture videos providing valuable insights into techniques, resources, and stay up to date with the latest advancements in the field.
Cell Culture Selection Tools
Find the right cell culture products for your research with these selection tools. Search for media, FBS, plastics, transfection reagents, and more.
Support
Cell Culture & Transfection Support Center
Find technical support recommendations for your cell culture and transfection workflows, including tips for experimental setup and in-depth troubleshooting help.
Need technical support?
Contact our expert team for technical and application support of Laboratory Products.
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