A variety of plates in different well offerings

Culture plate selection guides

Choose from a wide range of styles, sizes, and surfaces to suit a variety of cell culture applications. Thermo Scientific Nunc cell culture plates and well plates are available in more than 100 combinations of format and surface, designed to help promote healthy cells and reproducible results. Lot-to-lot cell line testing helps to ensure consistency.

A variety of plates in different well offerings

Plates are available in solid (including black and white, for use in fluorescence or luminescence studies respectively) and clear plastics.

Solid plates are fully molded well plates in either clear polystyrene. Clear plates have excellent imaging properties for magnification up to 40x. Optical bottom plates with thin polystyrene film or coverglass bottoms are engineered for superior imaging properties to provide low autofluorescence, absence of a halo effect, improved optical clarity, and high signal-to-noise ratios.

To choose the best plate for you, please see below guides or the plate selection tool.

 Explore plates by detection technology

Cell culture plate and multiwell plate sizes

Nunc plates for cell culture are available in a myriad of sizes, colors, and surface treatments. To help your selection process, these products have been categorized in different ways. Above, use our cell culture plate selection tool and find  plates by detection technology or plates by surface types guides. Below, plates are categorized by size and then by plastic. On each tab, find plate features and the ability to order the products that suit your needs best.

Four, rectangular single well plates

Polystyrene single-well plates come as both OmniTrays, optimized for screen libraries and DNA hybridization, as well as a standard ANSI-dimensioned rectangular plate optimized for use with molecular biology robotic instrumentation. OmniTrays come with both untreated and Nunclon Delta treated surfaces.

Well shapeRectangle
Surface area/well84 cm2
Large packaging available?No
Nunclon Delta for adherent cells (Cat. No.)165218140156
Non-treated for suspension cells (Cat. No.)242811264728267060
A single four well plate

Nunc 4-well multidishes feature:

  • Polystyrene
  • Flat well bottoms for stackability
  • Gamma irradiation sterilization
  • Non-pyrogenic certification
Well shapeRectangleRound
Surface area/well21.8 cm21.9 cm2
Large packaging available?NoNo
Nunclon Delta for adherent cells (Cat. No.)167063176740
Non-treated for suspension cells (Cat. No.)267061179820
A single six well plate

Nunc 6-well multidishes feature:

  • Flat well bottoms for stackability
Well shapeRound
Surface area/well9.6 cm2
Large packaging available?NoYes
Nunclon Delta for adherent cells (Cat. No.)140675140685
Non-treated for suspension cells (Cat. No.)150239 
Nunclon Sphera for spheroid-organoid cultures (Cat. No.)174932 
UpCell for adherent culture plus trypsin-free harvesting (Cat. No.)174901 
Poly-D-lysine coating for finicky cells (Cat. No.)152035* 
Collagen I coating for finicky cells (Cat. No.)152034* 
Nunclon Vita for harvesting pluripotent stem cells (Cat. No.)145380 

*Aseptically manufactured

Nunc 8-well multidishes feature:

 

  • Gamma irradiation sterilization
  • Non-pyrogenic certification
Well shapeRectangle
Surface area/well10.5 cm2
Large packaging available?No
Nunclon Delta for adherent cells (Cat. No.)167064
Non-treated for suspension cells (Cat. No.)267062*

*Aseptically manufactured

A single twelve well plate

Nunc 12-well multidishes feature:

  • Polystyrene
  • Flat well bottoms for stackability
  • Gamma irradiation sterilization
  • Non-pyrogenic certification
Well shapeRound
Surface area/well3.5 cm2
Large packaging available?No
Nunclon Delta for adherent cells (Cat. No.)150628
Non-treated for suspension cells (Cat. No.)150200
Nunclon Sphera for spheroid-organoid cultures (Cat. No.)174931
UpCell for adherent culture plus trypsin-free harvesting (Cat. No.)174900
A single 24 well plate

Nunc 24-well multidishes feature:

  • Polystyrene
  • Flat well bottoms for stackability
  • Gamma irradiation sterilization
  • Non-pyrogenic certification
Well shapeRound
Surface area/well1.9 cm2
Large packaging available?NoYes
Nunclon Delta for adherent cells (Cat. No.)142475142485
Non-treated for suspension cells (Cat. No.)144530 
Nunclon Sphera for spheroid-organoid cultures (Cat. No.)174930 
UpCell for adherent culture plus trypsin-free harvesting (Cat. No.)174899 
Poly-D-lysine coating for finicky cells (Cat. No.)152025* 

*Aseptically manufactured

A single 48 well plate

Nunc 48-well multidishes feature:

  • Flat well bottoms for stackability
Well shapeRound
Surface area/well1.1 cm2
Large packaging available?NoYes
Nunclon Delta for adherent cells (Cat. No.)150687152640
Non-treated for suspension cells (Cat. No.)150787 
UpCell for adherent culture plus trypsin-free harvesting (Cat. No.)174898 
Four, rectangular single well plates

Polystyrene single-well plates come as both OmniTrays, optimized for screen libraries and DNA hybridization, as well as a standard ANSI-dimensioned rectangular plate optimized for use with molecular biology robotic instrumentation. OmniTrays come with both untreated and Nunclon Delta treated surfaces.

Well shapeRectangle
Surface area/well84 cm2
Large packaging available?No
Nunclon Delta for adherent cells (Cat. No.)165218140156
Non-treated for suspension cells (Cat. No.)242811264728267060
A single four well plate

Nunc 4-well multidishes feature:

  • Polystyrene
  • Flat well bottoms for stackability
  • Gamma irradiation sterilization
  • Non-pyrogenic certification
Well shapeRectangleRound
Surface area/well21.8 cm21.9 cm2
Large packaging available?NoNo
Nunclon Delta for adherent cells (Cat. No.)167063176740
Non-treated for suspension cells (Cat. No.)267061179820
A single six well plate

Nunc 6-well multidishes feature:

  • Flat well bottoms for stackability
Well shapeRound
Surface area/well9.6 cm2
Large packaging available?NoYes
Nunclon Delta for adherent cells (Cat. No.)140675140685
Non-treated for suspension cells (Cat. No.)150239 
Nunclon Sphera for spheroid-organoid cultures (Cat. No.)174932 
UpCell for adherent culture plus trypsin-free harvesting (Cat. No.)174901 
Poly-D-lysine coating for finicky cells (Cat. No.)152035* 
Collagen I coating for finicky cells (Cat. No.)152034* 
Nunclon Vita for harvesting pluripotent stem cells (Cat. No.)145380 

*Aseptically manufactured

Nunc 8-well multidishes feature:

 

  • Gamma irradiation sterilization
  • Non-pyrogenic certification
Well shapeRectangle
Surface area/well10.5 cm2
Large packaging available?No
Nunclon Delta for adherent cells (Cat. No.)167064
Non-treated for suspension cells (Cat. No.)267062*

*Aseptically manufactured

A single twelve well plate

Nunc 12-well multidishes feature:

  • Polystyrene
  • Flat well bottoms for stackability
  • Gamma irradiation sterilization
  • Non-pyrogenic certification
Well shapeRound
Surface area/well3.5 cm2
Large packaging available?No
Nunclon Delta for adherent cells (Cat. No.)150628
Non-treated for suspension cells (Cat. No.)150200
Nunclon Sphera for spheroid-organoid cultures (Cat. No.)174931
UpCell for adherent culture plus trypsin-free harvesting (Cat. No.)174900
A single 24 well plate

Nunc 24-well multidishes feature:

  • Polystyrene
  • Flat well bottoms for stackability
  • Gamma irradiation sterilization
  • Non-pyrogenic certification
Well shapeRound
Surface area/well1.9 cm2
Large packaging available?NoYes
Nunclon Delta for adherent cells (Cat. No.)142475142485
Non-treated for suspension cells (Cat. No.)144530 
Nunclon Sphera for spheroid-organoid cultures (Cat. No.)174930 
UpCell for adherent culture plus trypsin-free harvesting (Cat. No.)174899 
Poly-D-lysine coating for finicky cells (Cat. No.)152025* 

*Aseptically manufactured

A single 48 well plate

Nunc 48-well multidishes feature:

  • Flat well bottoms for stackability
Well shapeRound
Surface area/well1.1 cm2
Large packaging available?NoYes
Nunclon Delta for adherent cells (Cat. No.)150687152640
Non-treated for suspension cells (Cat. No.)150787 
UpCell for adherent culture plus trypsin-free harvesting (Cat. No.)174898 

Cell culture plate specifications

Some useful numbers such as surface area and volumes of dissociation solutions are given below for various size culture plates.

SizeSeeding density (cells /well)Cells at confluency (cells/well)Versene (mL** of 0.05% EDTA)Trypsin (mL** of 0.05% trypsin, 0.53 mM EDTA)Growth medium (mL**)
6-well0.3 x 1061.2 x 106111–3
12-well0.1 x 1060.5 x 1060.4–10.4–11–2
24-well0.05 x 1060.24 x 1060.2–0.30.2–0.30.5–1.0
48-well0.03 x 1060.12 x 1060.1–0.20.1–0.20.2–0.4

**Approximate volume


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