GeneArt™ Gene Synthesis Product Selection Guide | Thermo Fisher Scientific - US

Which technology is right for you?

	Do-it-yourself gene synthesis GeneArt Gene Synthesis Kit & CorrectASEenzyme	Uncloned gene fragments GeneArt Strings DNA Fragments	Full gene synthesis service GeneArt Gene Synthesis	Full vector construction service GeneArt Elements Vector Construction	Whole expression construct or pathway optimization GeneArt Elements Combinatorial Parts Assembly	Gene synthesis and protein production GeneArt Genes-to-Proteins
What we do	Provide all reagents to perform gene synthesis from oligo assembly to gene cloning, including CorrectASE™ enzyme	Synthesis of linear dsDNA fragments up to 3 kb	Synthesis of DNA of interest, cloning of DNA into standard vector or expression vector of choice (ready to use)	Synthesis of complete vectors using defined DNA parts with known sequence/function, in combination with custom sequences	Synthesis of multiple DNA part combinations cloned into a customer-specific vector	Expression and purification of proteins from mammalian cells as all-in-one solution, starting from only the electronic nucleotide sequence
Advantages	Full cost control Every step in your hands CorrectASE™ enzyme helps reduce oligo-based mutations	Affordable and fast Gene design flexibility Gene optimization function No physical template required Reliable technology available to clone or assemble your complete gene (e.g., GeneArt™ Type IIs Assembly Kits)	Convenient ordering Full sequence design flexibility Synthesis of large sequences (<100 kb) Full gene optimization No physical template required Synthesis of complex sequences (e.g., high/low GC content) Downstream services available	Predefined DNA parts from repository CAD-like design/ordering software No physical template required	DNA parts may be used multiple times All parts combinations are possible Identify the combination of parts that fits your needs No physical template required	All production steps from a single-source provider Experienced scientists Reliable and advanced expression systems (FreeStyle™ 293, CHO, and Expi293™) Expression-optimized genes Expression scales from mL to 25 L No physical template required
Hands-on time	High	Medium	Low	Low	Low	None
Deliverables	Kit (10 reactions)	200 ng linear dsDNA, uncloned (lyophilized)	5 µg lyophilized plasmid DNA	5 µg lyophilized plasmid DNA	Single construct delivery: 5 µg lyophilized plasmid DNA Pooled delivery: 30 µg of plasmid DNA and Glycerol stock of clone pool	Protein of interest and expression vector used for transfection
Production time*	N/A	5 business days for <1 kb 8 business days for 1–3 kb	9 business days for genes <1,200 bp 14 business days for genes <3,000 bp Express and SuperSPEED options are available	Project-specific	Project-specific	Typically 30 business days
Length	Recommended for up to 1 kb	Up to 3 kb	Up to 100 kb	Up to 12 kb vectors	Project-specific	N/A
Quality control	N/A	Bulk sequencing	100% sequence-verified	100% sequence-verified and IP check	Single construct delivery: 100% sequence verified Pooled delivery: Peer group sequencing	Detailed documentation, including Coomassie-stained PAGE gel and western blot
	Add to cart	Order now	Order now	Learn more	Learn more	Learn more

	Do-it-yourself gene synthesis GeneArt™ Gene Synthesis Kit & CorrectASE™ enzyme	Uncloned gene fragments GeneArt™ Strings™ DNA Fragments	Full gene synthesis service GeneArt™ Gene Synthesis	Full vector construction service GeneArt™ Elements™ Vector Construction	Whole expression construct or pathway optimization GeneArt™ Elements™ Combinatorial Parts Assembly	Gene synthesis and protein production GeneArt™ Genes-to-Proteins
What we do	Provide all reagents to perform gene synthesis from oligo assembly to gene cloning, including CorrectASE™ enzyme	Synthesis of linear dsDNA fragments up to 3 kb	Synthesis of DNA of interest, cloning of DNA into standard vector or expression vector of choice (ready to use)	Synthesis of complete vectors using defined DNA parts with known sequence/function, in combination with custom sequences	Synthesis of multiple DNA part combinations cloned into a customer-specific vector	Expression and purification of proteins from mammalian cells as all-in-one solution, starting from only the electronic nucleotide sequence
Advantages	Full cost control Every step in your hands CorrectASE™ enzyme helps reduce oligo-based mutations	Affordable and fast Gene design flexibility Gene optimization function No physical template required Reliable technology available to clone or assemble your complete gene (e.g., GeneArt™ Type IIs Assembly Kits)	Convenient ordering Full sequence design flexibility Synthesis of large sequences (<100 kb) Full gene optimization No physical template required Synthesis of complex sequences (e.g., high/low GC content) Downstream services available	Predefined DNA parts from repository CAD-like design/ordering software No physical template required	DNA parts may be used multiple times All parts combinations are possible Identify the combination of parts that fits your needs No physical template required	All production steps from a single-source provider Experienced scientists Reliable and advanced expression systems (FreeStyle™ 293, CHO, and Expi293™) Expression-optimized genes Expression scales from mL to 25 L No physical template required
Hands-on time	High	Medium	Low	Low	Low	None
Deliverables	Kit (10 reactions)	200 ng linear dsDNA, uncloned (lyophilized)	5 µg lyophilized plasmid DNA	5 µg lyophilized plasmid DNA	Single construct delivery: 5 µg lyophilized plasmid DNA Pooled delivery: 30 µg of plasmid DNA and Glycerol stock of clone pool	Protein of interest and expression vector used for transfection
Production time*	N/A	5 business days for <1 kb 8 business days for 1–3 kb	9 business days for genes <1,200 bp 12 business days for genes <3,000 bp Express and SuperSPEED options are available	Project-specific	Project-specific	Typically 30 business days
Length	Recommended for up to 1 kb	Up to 3 kb	Up to 100 kb	Up to 12 kb vectors	Project-specific	N/A
Quality control	N/A	Bulk sequencing	100% sequence-verified	100% sequence-verified and IP check	Single construct delivery: 100% sequence verified Pooled delivery: Peer group sequencing	Detailed documentation, including Coomassie-stained PAGE gel and western blot
	Add to cart	Order now	Order now	Learn more	Learn more	Learn more

*Number of days for production and to initiate the shipping process, NOT the number of days it will take to receive the delivery. Delivery time varies depending on location.

For Research Use Only. Not for use in diagnostic procedures.

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