AccuPrime Taq DNA Polymerase

Invitrogen AccuPrime DNA polymerases utilize AccuPrime accessory proteins for improved PCR fidelity, yield, and specificity. AccuPrime technology is available in Taq, Pfx, and GC-Rich formulations.

Overview of AccuPrime technology

The thermostable AccuPrime accessory proteins enhance specific primer–template hybridization during every cycle of PCR, preventing mispriming and improving PCR specificity and yield. In addition, AccuPrime enzymes are designed with antibodies that are specific to the DNA polymerase, inhibiting its activity at room temperature and providing an automatic hot start.

Mechanism of AccuPrime hot-start process in PCR
How AccuPrime technology works.


Comparison of Platinum and AccuPrime DNA polymerases

Properties of Platinum DNA polymerases and AccuPrime DNA polymerases are compared for their use in high-fidelity PCR, hot-start PCR, and GC-rich PCR.

 Platinum SuperFi II DNA PolymeraseAccuPrime Taq DNA Polymerase, High FidelityAccuPrime Pfx DNA Polymerase
Fidelity (vs. Taq)>300x9x26x
Hot-start modification   
Universal primer annealing NoNo
Amplification range20 kb*≤20 kb (with Buffer II)≤12 kb
Amplicon overhangBlunt3′A and bluntBlunt
GC-rich format NoNo
Inhibitor tolerance NoNo
DNA synthesis rate15–30 sec/kb60 sec/kb60 sec/kb
Residual bacterial gDNA≤1 copy (per 50 μL rxn)Not testedNot tested
Buffer systemOne optimized buffer
(GC enhancer not requered)
Two buffers
(based on template type)
One buffer
Product formatsStand alone:
Colorless

Master mix:
Colorless
Green
Stand alone:
Colorless
Stand alone:
Colorless

* Amplification up to 40 kb is possible depending on complexity of template DNA
† Contains green buffer that includes density reagent and two tracking dyes for direct gel loading

 Platinum II Taq DNA PolymeraseAccuPrime Taq DNA Polymerase System
Hot-start modification  
Fidelity (vs. Taq)1x2x
Amplification range≤5 kb≤5 kb
Inhibitor tolerance No
DNA synthesis rate15 sec/kb60 sec/kb
Amplicon overhang3′A3′A
Universal primer annealing No
Residual bacterial gDNA≤1 copy/enzyme unitNot tested
GC-rich format No
MultiplexingUp to 15Up to 20
Buffer systemOne optimized buffer
(GC enhancer supplied in a separate vial)
Two buffers
(based on template type)
Product formatsStand alone:
 Colorless
Master mix:
 Colorless
 Green
Stand alone:
 Colorless

† Contains green buffer that includes density reagent and two tracking dyes for direct gel loading

 Platinum SuperFi II DNA PolymerasePlatinum II Taq DNA PolymeraseAccuPrime GC-Rich DNA Polymerase
GC-rich amplification>65% GC>65% GC>65% GC
Hot-start modification  No
Fidelity (vs. Taq)>300x1x2x
Amplification range20 kb*≤5 kb≤5 kb
Amplicon overhangBlunt3′ABlunt
Inhibitor tolerance  No
DNA synthesis rate15–30 sec/kb15 sec/kb60 sec/kb
Universal primer annealing  No
Residual bacterial gDNA≤1 copy (per 50 μL rxn)≤1 copy (per 50 μL rxn)Not tested
Buffer systemOne optimized buffer
(GC enhancer not required)
One optimized buffer
(GC enhancer supplied in a separate vial)
Two-buffer system
(based on GC%)
Product formatsStand alone:
 Colorless
 
Master mix:
 Colorless
 Green
Stand alone:
Colorless

Master mix:
 Colorless
 Green
Stand alone:
 Colorless

* Amplification up to 40 kb is possible depending on complexity of template DNA
† Contains green buffer that includes density reagent and two tracking dyes for direct gel loading

 Platinum SuperFi II DNA PolymeraseAccuPrime Taq DNA Polymerase, High FidelityAccuPrime Pfx DNA Polymerase
Fidelity (vs. Taq)>300x9x26x
Hot-start modification   
Universal primer annealing NoNo
Amplification range20 kb*≤20 kb (with Buffer II)≤12 kb
Amplicon overhangBlunt3′A and bluntBlunt
GC-rich format NoNo
Inhibitor tolerance NoNo
DNA synthesis rate15–30 sec/kb60 sec/kb60 sec/kb
Residual bacterial gDNA≤1 copy (per 50 μL rxn)Not testedNot tested
Buffer systemOne optimized buffer
(GC enhancer not requered)
Two buffers
(based on template type)
One buffer
Product formatsStand alone:
Colorless

Master mix:
Colorless
Green
Stand alone:
Colorless
Stand alone:
Colorless

* Amplification up to 40 kb is possible depending on complexity of template DNA
† Contains green buffer that includes density reagent and two tracking dyes for direct gel loading

 Platinum II Taq DNA PolymeraseAccuPrime Taq DNA Polymerase System
Hot-start modification  
Fidelity (vs. Taq)1x2x
Amplification range≤5 kb≤5 kb
Inhibitor tolerance No
DNA synthesis rate15 sec/kb60 sec/kb
Amplicon overhang3′A3′A
Universal primer annealing No
Residual bacterial gDNA≤1 copy/enzyme unitNot tested
GC-rich format No
MultiplexingUp to 15Up to 20
Buffer systemOne optimized buffer
(GC enhancer supplied in a separate vial)
Two buffers
(based on template type)
Product formatsStand alone:
 Colorless
Master mix:
 Colorless
 Green
Stand alone:
 Colorless

† Contains green buffer that includes density reagent and two tracking dyes for direct gel loading

 Platinum SuperFi II DNA PolymerasePlatinum II Taq DNA PolymeraseAccuPrime GC-Rich DNA Polymerase
GC-rich amplification>65% GC>65% GC>65% GC
Hot-start modification  No
Fidelity (vs. Taq)>300x1x2x
Amplification range20 kb*≤5 kb≤5 kb
Amplicon overhangBlunt3′ABlunt
Inhibitor tolerance  No
DNA synthesis rate15–30 sec/kb15 sec/kb60 sec/kb
Universal primer annealing  No
Residual bacterial gDNA≤1 copy (per 50 μL rxn)≤1 copy (per 50 μL rxn)Not tested
Buffer systemOne optimized buffer
(GC enhancer not required)
One optimized buffer
(GC enhancer supplied in a separate vial)
Two-buffer system
(based on GC%)
Product formatsStand alone:
 Colorless
 
Master mix:
 Colorless
 Green
Stand alone:
Colorless

Master mix:
 Colorless
 Green
Stand alone:
 Colorless

* Amplification up to 40 kb is possible depending on complexity of template DNA
† Contains green buffer that includes density reagent and two tracking dyes for direct gel loading


AccuPrime DNA polymerase products

The following table summarizes AccuPrime DNA polymerases and formats available for PCR.

 AccuPrime Taq DNA Polymerase, High FidelityAccuPrime Pfx DNA PolymeraseAccuPrime GC-Rich DNA PolymeraseAccuPrime Taq DNA Polymerase System
DNA polymerase(s)Blend of Taq DNA polymerase and proofreading GB-D enzyme from Pyrococcus speciesRecombinant KOD enzyme from Thermococcus speciesDNA polymerase cloned from Pyrolobus fumariusAccuPrime Taq DNA polymerase
Hot-start modification  No 
Enhanced specificity with AccuPrime accessory proteins    
Fidelity
(vs. Taq)
9x26x2x2x
Amplicon overhang3′A and bluntBluntBlunt3′A
Product formatsStand-alone enzyme with the two-buffer system:
Buffer I for plasmids, cDNA, and λ DNA;
Buffer II for gDNA.
Stand-alone enzymeStand-alone enzyme with the two-buffer system:
Buffer A for GC-rich gDNA targets;
Buffer B for non–GC-rich gDNA, cDNA, plasmids, and λ DNA.
Stand-alone enzyme with the two-buffer system:
Buffer I for small gDNA amplicons (≤200 bp), cDNA, and plasmids;
Buffer II for gDNA (200 bp–4 kb).


Ordering information

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