Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
TaqMan® Mutation Detection Assays detect and measure somatic mutations in genes that are associated with cancer. These research use only assays are powered by competitive allele-specific TaqMan® PCR technology, also known as castPCR™ technology. The castPCR™ technology is highly specific and sensitive, and it can detect and quantitate rare amounts of mutated DNA in a sample that contains large amounts of normal, wild type DNA.
Isolate and purify genomic DNA.
![]() | BloodPrep® DNA Chemistry for Cultured Cells and Blood
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Select appropriate TaqMan® Mutation Detection Assay products.
![]() | TaqMan® Mutation Detection Assays |
Prepare reaction plate and amplify DNA using sequence specific primers and TaqMan® probe on an Applied Biosystems® Real-Time PCR System or thermal cycler.
![]() | TaqMan® Genotyping Master Mix
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Instruments & Accessories | ||
![]() | Veriti® 96-Well Thermal Cycler
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![]() | Dual 96-Well GeneAmp® PCR System 9700
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![]() | StepOnePlus™ Real-Time PCR System | |
![]() | 7500 Fast Real-Time PCR System
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![]() | 7500 Real-Time PCR System | |
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![]() | 7900HT Fast Real-Time PCR System
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![]() | ViiA™ 7 Real-Time PCR System
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Perform an endpoint plate read using Applied Biosystems® Real-Time PCR Systems.
Instruments & Accessories | ||
![]() | StepOnePlus™ Real-Time PCR System | |
![]() | 7500 Fast Real-Time PCR System
| |
|
| |
| ||
![]() | 7900HT Fast Real-Time PCR System
| |
![]() | ViiA™ 7 Real-Time PCR System
|
The plotted fluorescence signals indicate which alleles are in each sample. Make manual allele calls or review automatic allele calls, and then convert allele calls to genotypes.
Mutation Detector™ Software
Mutation Detector™ Software helps determine the mutation status and percent mutation for samples from data collected on supported Applied Biosystems® Real-Time PCR Systems.
Isolate and purify genomic DNA.
![]() | BloodPrep® DNA Chemistry for Cultured Cells and Blood
|
Select appropriate TaqMan® Mutation Detection Assay products.
![]() | TaqMan® Mutation Detection Assays |
Prepare reaction plate and amplify DNA using sequence specific primers and TaqMan® probe on an Applied Biosystems® Real-Time PCR System or thermal cycler.
![]() | TaqMan® Genotyping Master Mix
| |
Instruments & Accessories | ||
![]() | Veriti® 96-Well Thermal Cycler
| |
![]() | Dual 96-Well GeneAmp® PCR System 9700
| |
![]() | StepOnePlus™ Real-Time PCR System | |
![]() | 7500 Fast Real-Time PCR System
| |
|
| |
![]() | 7500 Real-Time PCR System | |
| ||
![]() | 7900HT Fast Real-Time PCR System
| |
![]() | ViiA™ 7 Real-Time PCR System
|
Perform an endpoint plate read using Applied Biosystems® Real-Time PCR Systems.
Instruments & Accessories | ||
![]() | StepOnePlus™ Real-Time PCR System | |
![]() | 7500 Fast Real-Time PCR System
| |
|
| |
| ||
![]() | 7900HT Fast Real-Time PCR System
| |
![]() | ViiA™ 7 Real-Time PCR System
|
The plotted fluorescence signals indicate which alleles are in each sample. Make manual allele calls or review automatic allele calls, and then convert allele calls to genotypes.
Mutation Detector™ Software
Mutation Detector™ Software helps determine the mutation status and percent mutation for samples from data collected on supported Applied Biosystems® Real-Time PCR Systems.