Articles that detail flow cytometry applications

Flow Cytometry Application Notes

The articles listed below comprise our application notes, scientific posters, and BioProbes Journal articles collections. These resources give overviews of specific flow cytometry applications and as well as experimental design details and data and results. 

Resource library collections


Featured articles

Copper-safe Click-iT Plus EdU Proliferation Assay: Improved compatibility with simultaneous phycobiliprotein and fluorescent protein detection

This scientific poster demonstrates how the Click-iT Plus EdU Proliferation assay can be used with classic fluorescent proteins and phycobiliproteins for cell proliferation detection, eliminating the need for using cumbersome antibody-based Anti-BrdU assays.

Multiparameter analysis of murine regulatory T cells and dendritic cells with the Attune NxT Flow Cytometer

This application note describes the use of the Attune NxT Flow Cytometer for 4-laser and 10-color immunophenotyping analysis of stained mouse splenocytes using a fixation and permeabilization protocol.


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Type Title Category
Application note (2018)  High-throughput automation with the Attune NxT Autosampler: consistent results across all wells and across plates  Attune/Attune NxT Flow Cytometer, Autosampler
Application note (2018)  A guide to analyze Attune NxT FCS data files in FCS Express 6 Software  Attune/Attune NxT Flow Cytometer, Attune NxT software, flow cytometry
Application note (2017) Recommendations for accurate concentration measurements on the Attune NxT Flow Cytometer Attune/Attune NxT Flow Cytometer, flow cytometry, cell concentration, cell counting
Application note (2017) Flow cytometry analysis of transcription factor expression during differentiation of hPSC-derived cardiomyocytes Attune/Attune NxT Flow Cytometer, cell differentiation, flow cytometry, multicolor flow cytometry, stem cell research
Application note (2017) Validated 10-color T cell panel using the Attune NxT Flow Cytometer antibodies, Attune/Attune NxT Flow Cytometer, flow cytometry, immunology, immunophenotyping, fluorescent dyes, multicolor flow cytometry
Application note (2017)  Flow cytometry analysis of dose response for apoptosis induction  apoptosis, Attune NxT, Autosampler, flow cytometry, cell toxicity, CellEvent Caspase-3/7 Green 
Application note (2017)  Detection of platelets in whole blood using the Attune NxT Flow Cytometer  Attune NxT, flow cytometer, platelets, immunophenotyping, no-wash, no-lyse
Application note (2015) Attune NxT Flow Cytometer for 6-color immunophenotyping analysis of stained human whole blood using a no-lyse, no-wash protocol, with no compensation Attune NxT, flow cytometer, immunophenotyping, multicolor flow cytometry
Application note (2015) Detection of murine regulatory T cells on the Attune NxT Flow Cytometer Attune NxT, flow cytometer, immunophenotyping, multicolor flow cytometry
Application note (2015) Multiparameter analysis of murine regulatory T cells and dendritic cells with the Attune NxT Flow Cytometer Attune NxT, flow cytometer, immunophenotyping, multicolor flow cytometry
Application note (2015) Ten-parameter immunophenotyping of human lysed whole blood with the Attune NxT Flow Cytometer allows identification of multiple T cell subsets and myeloid cells Attune NxT, flow cytometer, immunophenotyping, multicolor flow cytometry
Application note (2015)  13-parameter immunophenotyping of human lysed whole blood with the Attune NxT Flow Cytometer allows for the identification of B cells, NK cells, multiple T cell subsets and myeloid cells  Alexa Fluor, antibodies, Attune NxT, compensation, flow cytometer, fluorescent dyes, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot
Application note (2015)  Live/dead algal analysis with the Attune NxT Flow Cytometer  algae, Attune NxT, flow cytometer, flow cytometry assays, fluorescent dyes, microbiology, viability
Application note (2015)  No-wash, no-lyse detection of phagocytic cells via a pHrodo BioParticles functional assay in human whole blood on the Attune NxT Flow Cytometer  antibodies, Attune NxT, flow cytometer, flow cytometry sample preparation, fluorescent dyes, immunology, immunophenotyping, particles, pH detection
BioProbes article (2018) Immuno-oncology: Advances in Basic Research and Translational Medicine. With a focus on immune checkpoint inhibitors and T cell immunotherapy antibodies, ELISA, flow cytometry, fluorescence microscopy/fluorescence imaging, high content analysis (HCA), immunohistochemistry (IHC), immunology, immuno-oncology, immunophenotyping, Luminex, ProCartaPlex, spheroids
BioProbes article (2018) Flow cytometric assay for simultaneous assessment of HIV RNA and Gag protein. Single-cell characterization of viral translation-competent reservoirs in HIV-infected individuals antibodies and proteins, flow cytometry, HIV, immunology, in situ hybridization, PrimeFlow, protein expression, RNA detection
BioProbes article (2018) A comprehensive resource for state-of-the-art flow cytometry methods. Guidelines for the use of flow cytometry and cell sorting in immunological studies antibodies and proteins, cell counting, cell cycle, cell proliferation, flow cytometer calibration, flow cytometry, flow cytometry compensation, flow cytometry sample preparation, immunology, immunophenotyping, viability
BioProbes article (2017) Harness immune checkpoints to combat tumors—Immune checkpoint antibodies for flow cytometry, IHC, and functional bioassays antibodies, cancer, flow cytometry, immunofluorescence (IF), immunohistochemistry (IHC)
BioProbes article (2017) Bright fluorescent polymer dyes for the violet laser—Super Bright antibody conjugates antibodies, antibody labeling, Attune NxT, flow cytometry, fluorescence, immunophenotyping, multicolor flow cytometry, Super Bright
BioProbes article (2017) Powering up drug discovery—Cancer biology applications using the Attune NxT Flow Cytometer apoptosis, Attune NxT, cancer, flow cytometer, fluorescence, multicolor flow cytometry, viability
BioProbes article (2017) Evaluate both RNA and protein targets in single cells—PrimeFlow RNA assay for detecting RNA targets by flow cytometry Alexa Fluor, flow cytometry, fluorescence, in situ hybridization, PrimeFlow, RNA
BioProbes article (2017) Journal club: measurement and characterization of apoptosis by flow cytometry apoptosis, caspase substrates, flow cytometry, fluorescence, viability
BioProbes article (2017) T lymphocyte immunophenotyping—14-color flow cytometry panel featuring Super Bright antibody conjugates antibodies, Attune NxT, immunology, immunophenotyping, multicolor flow cytometry, Super Bright
BioProbes article (2017) An evaluation of dyes for tracking cell proliferation by flow and image cytometry cell proliferation, cell tracking, flow cytometry, fluorescence microscopy/fluorescence imaging
BioProbes article (2016) High-speed cell counting with the Attune NxT Flow Cytometer—Accurately measure cell concentrations by flow cytometry Attune NxT, bacteria, cell concentration, cell counting, flow cytometer, immunophenotyping, microbiology, viability
BioProbes article (2016) A guide to studying cell proliferation by generational tracing—Tracking cell division with flow cytometry CellTrace, cell cycle, cell proliferation, cell tracking, flow cytometry
BioProbes article (2016) A complete suite of reagents, from cas9 delivery tools to cell function Assays—The CRISPR-cas9 system for genome editing CRISPR, flow cytometry assays, fluorescence, fluorescence microscopy/fluorescence imaging, high content analysis, western blotting
BioProbes article (2016) Transcription factor expression during differentiation of hPSC-derived cardiomyocytes—A multiparametric approach using the Attune NxT Flow Cytometer Attune NxT, flow cytometer, fluorescence, large cell analysis, rare event detection, stem cell research
BioProbes article (2016) Optimized multicolor immunofluorescence panels (OMIPs)—Jump-start your experimental design with published antibody and reagent panels flow cytometry, fluorescence, immunophenotyping, multicolor flow cytometry
BioProbes article (2015) Flow cytometry panel design: The basics flow cytometry, fluorescence, multicolor flow cytometry
BioProbes article (2015) Isolation and characterization of exosomes using magnetic beads antibodies, exosomes, flow cytometry, fluorescence, immunophenotyping, multiplex ELISA/Luminex,  particles, protein blotting, western detection
BioProbes article (2015) Journal Club—Multiparameter detection of early apoptosis markers without compensation apoptosis, Attune NxT, cell structure-mitochondria, flow cytometry compensation, fluorescence, immunophenotyping
BioProbes article (2015) Multiparameter detection of fluorescent protein expression with the Attune NxT Flow Cytometer Attune NxT, flow cytometer, flow cytometry assays, fluorescence, fluorescent proteins, multicolor flow cytometry
BioProbes article (2015) No-compensation immunophenotyping using a four-laser flow cytometer Attune NxT, flow cytometer, fluorescence, immunophenotyping, multicolor flow cytometry
BioProbes article (2015) No-wash, no-lyse assays for the Attune NxT Flow Cytometer Attune NxT, endocytosis, flow cytometer, flow cytometry assays, fluorescence, immunophenotyping, multicolor flow cytometry, phagocytosis
BioProbes article (2015) The next generation in flow cytometry—The Attune NxT Flow Cytometer antibodies, Attune NxT, flow cytometer, fluorescence
BioProbes article (2015) Tools and strategies for rare-event detection using flow cytometry Attune NxT, flow cytometer, fluorescence, rare cell detection, rare event detection
BioProbes article (2014) Detect pH inside of a live cell—pHrodo pH sensors for detecting cytosolic and vesicle pH flow cytometry, fluorescence microscopy/fluorescence imaging, microplate reader, pHrodo
BioProbes article (2014) Multiplex your cell proliferation assay with GFP, RFP, and R-PE probes—Click-iT Plus EdU Proliferation Kits for imaging and flow cytometry cell proliferation, Click-iT, cell proliferation, multicolor flow cytometry
BioProbes article (2014) Obtain the highest-quality data your system can deliver—Fluorescent microspheres for calibrating microscopes and flow cytometers calibration, cell counting, flow cytometer, fluorescence microscopy/fluorescence imaging, microscope calibration, particles
BioProbes article (2014) Superior live-cell tracing, generation after generation—The CellTrace Far Red Cell Proliferation Kit cell proliferation, CellTrace, flow cytometry, fluorescence
BioProbes article (2014) The next generation in acoustic focusing cytometry is here—The Attune NxT Acoustic Focusing Cytometer Attune NxT, flow cytometer
BioProbes article (2013) Checking vital signs: Don't let dead cells mislead you—Molecular Probes cell health assays for flow cytometry apoptosis, caspase substrate, cell cycle, cell proliferation, cell tracking, Click-iT, flow cytometry, fluorescence, immunophenotyping, oxidative stress, viability,
BioProbes article (2013) Oxidative stress from a cell's point of view—From CellROX ROS sensors to Image-iT and Click-iT lipid peroxidation detection kits CellROX, Click-iT, flow cytometry, fluorescence microscopy/fluorescence imaging, oxidative stress
BioProbes article (2012) Tracking internalization in live cells with pHrodo dyes endocytosis/phagocytosis, flow cytometry, fluorescence microscopy/fluorescence imaging, Internalization, pHrodo
BioProbes article (2011) Tracking cells through time and space—Choose from fluorescent Dyes, Qdot nanocrystals, and targeted fluorescent proteins CellTrace, cell tracking, fluorescence microscopy/fluorescence imaging, fluorescent proteins,  live cells
BioProbes article (2011) Click-iT EdU Flow Cytometry Kits— Simplifying cell proliferation analysis cell proliferation, Click-iT, flow cytometry, fluorescence
BioProbes article (2010) A ratiometric approach to apoptosis detection by flow cytometry apoptosis, flow cytometry, fluorescence
BioProbes article (2010) Advanced cell proliferation analysis Attune NxT cell proliferation, cell tracking, flow cytometry, fluorescence
BioProbes article (2010) Optimal pairing of dyes and antibodies for flow cytometry antibodies, fluorescence, multicolor flow cytometry, particles Qdot
BioProbes article (2010) Dead cell identification in flow cytometry flow cytometry, fluorescence, immunophenotyping, viability
BioProbes article (2009)  A flow cytometry–based method for isolating neural stem cells  Alexa Fluor, cell isolation, flow cytometry, fluorescent dyes, neuroscience, stem cell research
BioProbes article (2009)  A growing list of tools for the violet laser  Alexa Fluor, apoptosis, cell cycle, Click-iT, flow cytometry, fluorescent dyes, immunophenotyping, nanocrystals, Qdot, viability
BioProbes article (2009)  Accurate compensation is as easy as AbC  calibration, compensation, fluorescent dyes, immunophenotyping, multicolor flow cytometry, particles
BioProbes article (2009)  Beyond classification—Click-iT EDU outperforms BRDU across all species  Alexa Fluor, cell cycle, cell proliferation, Click-iT, flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, nucleic acid detection, nucleic acid quantitation, nucleosides, sample preparation
BioProbes article (2009)  Dead cell stains in flow cytometry: A comprehensive analysis  apoptosis, fluorescent dyes, viability
BioProbes article (2009)  Is it time to go direct? IgG antibody labeling methods as versatile as your imagination  Alexa Fluor, antibody labeling, flow cytometry, fluorescent dyes, immunocytochemistry (ICC), immunohistochemistry (IHC), nanocrystals, Qdot, Zenon
BioProbes article (2009)  Isolate T cells directly from whole blood  cell isolation, cell proliferation, Click-iT, flow cytometry, fluorescent dyes, immunology, magnetic beads
BioProbes article (2009)  Isolation and expansion of mouse Treg cells using Dynabeads technology  Alexa Fluor, cell expansion, cell isolation, flow cytometry, fluorescent dyes, immunology, magnetic beads
BioProbes article (2009)  Traffic lights: Illuminating internalization pathways  drug discovery, flow cytometry, fluorescent dyes, particles, pH detection, phagocytosis
BioProbes article (2009)  Nanotechnology meets cellular analysis  cell structure-all, flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, media, nanocrystals, Qdot, sample stability
BioProbes article (2009)  Near-infrared dye for live cell cycle analysis  cell cycle, flow cytometry, fluorescent dyes, live-cell imaging, nucleic acid detection
BioProbes article (2009)  Novel solid-phase antibody labeling  Alexa Fluor, antibody labeling, flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, sample preparation, Zenon
BioProbes article (2008)  IgG antibody labeling from A to Z—Alexa Fluor to Zenon IgG Antibody Labeling Kits for imaging and flow cytometry  Alexa Fluor, antibody labeling, flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, protein labeling, Zenon
BioProbes article (2008)  Putting regulatory T cells to work—Isolate and expand CD4+ CD25+ Treg cells using Dynabeads magnetic separation technology  cell expansion, cell isolation, flow cytometry, fluorescent dyes, immunology, magnetic beads, reagents
BioProbes article (2008)  Seeing green—Alexa Fluor 488 conjugates outshine the rest  Alexa Fluor, flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, sample stability
BioProbes article (2008)  Single-step isolation of functional human NK cells  antibodies, cell isolation, flow cytometry, fluorescent dyes, immunology, magnetic beads, sample preparation
BioProbes article (2008)  The picture of cell health  Alexa Fluor, cell health, cell proliferation, Click-iT, fluorescent dyes, flow cytometry, fluorescence microscopy/fluorescence imaging, high content analysis, microplate reader, nucleic acid quantitation, nucleosides, reagents
BioProbes article (2008)  Using Qdot nanocrystal primary antibody conjugates in flow cytometry  fluorescent dyes, multicolor flow cytometry, nanocrystals, Qdot, sample preparation, set up
BioProbes article (2007)  A new approach to cell-based multiplexing expands the drug screening capabilities of flow cytometry  Alexa Fluor, drug discovery, flow cytometry, fluorescent dyes, high content analysis, sample preparation
BioProbes article (2007)  Breakthrough cell proliferation assay for flow cytometry  Alexa Fluor, cell proliferation, Click-iT, flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, nucleic acid quantitation
BioProbes article (2007)  Dead-cell discrimination in a different light  flow cytometry, fluorescent dyes, viability
BioProbes article (2007)  Improved results are just a click away  Alexa Fluor, cell proliferation, Click-iT, flow cytometry, fluorescent dyes,  fluorescence microscopy/fluorescence imaging, high content analysis, in vivo imaging, multicolor flow cytometry, nucleic acid quantitation, nucleosides
BioProbes article (2007)  Investigating apoptosis-induced loss of SYTO dye fluorescence  apoptosis, cell structure-mitochondria, enzyme activity detection, flow cytometry, fluorescent dyes
BioProbes article (2007)  Looking for apoptosis?  antibodies, apoptosis, colorimetric, ELISA, flow cytometry, fluorescence, fluorescent dyes, immunocytochemistry (ICC), protein detection, western blotting
BioProbes article (2007)  Multiparametric approaches for studying apoptosis  Alexa Fluor, antibodies, apoptosis, cell structure-mitochondria, cell structure-plasma membrane, enzyme activity detection, flow cytometry, fluorescent dyes, viability
BioProbes article (2007)  New applications for Vybrant DyeCycle stains  Alexa Fluor, cell cycle, cell isolation, drug discovery, flow cytometry, fluorescent dyes, high content analysis, live-cell imaging, multicolor flow cytometry, stem cell research
BioProbes article (2007)  Putting the pH in phagocytosis  fluorescent dyes, fluorescence microscopy/fluorescence imaging, microplate reader, multicolor flow cytometry, pH detection, phagocytosis, reagents, sample preparation
BioProbes article (2007)  Quantum dots as replacements for tandem dyes in flow cytometry  fluorescent dyes, multicolor flow cytometry, nanocrystals, protein labeling, Qdot
BioProbes article (2007)  Simultaneously visualize surface and intracellular markers  antibody labeling, cell fixatives, flow cytometry, fluorescent dyes, sample preparation, Zenon
BioProbes article (2007)  Streptavidin conjugates of R-phycoerythrin—detection workhorses  flow cytometry, fluorescent proteins, Luminex, microarrays, multiplex ELISA/Luminex, particles, protein labeling
BioProbes article (2007)  The latest in stem cell research  antibodies, antibody labeling, cell differentiation, cell expansion, flow cytometry, fluorescent dyes, growth factors, fluorescence microscopy/fluorescence imaging, stem cell research
BioProbes article (2007)  The use of Qdot nanocrystals in flow cytometry  flow cytometry, fluorescent dyes, multicolor flow cytometry, nanocrystals, protein labeling, Qdot, viability
BioProbes article (2006)  A guide to antibody labeling and detection  Alexa Fluor, antibody labeling, flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, nanocrystals, Qdot, Zenon
BioProbes article (2006)  Analyzing cell culture health and concentration  apoptosis, cell counting, fluorescent dyes, flow cytometry, fluorescence microscopy/fluorescence imaging, microplate reader, particles, viability
BioProbes article (2006)  Multicolor immunodetection using the violet laser  Alexa Fluor, antibody labeling, flow cytometry, fluorescent dyes, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot
Blog post Clog resistance of non-pressure based flow cytometers Attune NxT, flow cytometer
Blog post Super Bright conjugates for flow cytometry antibodies, Attune NxT, flow cytometry, immunophenotyping, multicolor flow cytometry, Super Bright
Blog post What are event rates and should you always trust the “Maximum Event Rate” of your flow cytometer? Attune NxT, flow cytometer, instrument set-up
Blog post Flow cytometer fluidics and why they are not all the same Attune NxT, flow cytometer, instrument set-up
Fluorophore Pacific Blue dye antibody conjugates, flow cytometry, immunophenotyping, violet laser
Fluorophore Pacific Green dye antibody conjugates, flow cytometry, immunophenotyping, violet laser
Fluorophore Pacific Orange dye antibody conjugates, flow cytometry, immunophenotyping, violet laser
Fluorophore Super Bright 436 dye antibody conjugates, flow cytometry, immunophenotyping, tandem polymer dyes, violet laser
Fluorophore Super Bright 600 dye antibody conjugates, flow cytometry, immunophenotyping, tandem polymer dyes, violet laser
Fluorophore Super Bright 645 dye antibody conjugates, flow cytometry, immunophenotyping, tandem polymer dyes, violet laser
Fluorophore Super Bright 702 dye antibody conjugates, flow cytometry, immunophenotyping, tandem polymer dyes, violet laser
Fluorophore Allophycocyanin (APC) antibody conjugates, flow cytometry, immunophenotyping, phycobiliproteins, tandem dyes
Fluorophore R-phycoerythrin (R-PE) antibody conjugates, flow cytometry, immunophenotyping, phycobiliproteins, tandem dyes
Fluorophore Cyan fluorescent protein (CFP) flow cytometry, fluorescent proteins
Fluorophore Green fluorescent protein (GFP) flow cytometry, fluorescent proteins
Fluorophore Red fluorescent protein (RFP) flow cytometry, fluorescent proteins
Fluorophore Fluorophore selection guide antibody conjugates, flow cytometry, immunophenotyping
Immunology information B cell activation functional assay immunology
Immunology information Costimulatory and inhibitory interactions control T cell response immunology, stimulation, T cells
Immunology information Foxp3 overview flow cytometry, Foxp3,intracellular staining
Immunology information Innate immunity overview immunology
Immunology information Innate lymphoid cells overview immunology
Immunology information Microbiome (Microbiota) overview immunology
Immunology information Natural killer cells overview immunology
Immunology information T cell activation functional assay immunology
Immunology information T follicular helper differentiation functional assay immunology
Immunology information T reg differentiation functional assay immunology
Immunology information T regulatory cells overview immunology
Immunology information Th1 differentiation functional assay immunology
Immunology information Th17 cells overview immunology
Immunology information Th17 differentiation functional assay immunology
Immunology information Th2 differentiation functional assay immunology
Immunology information Th22 differentiation functional assay immunology
Immunology information Th9 differentiation functional assay immunology
Immunology information Toll-like receptor overview  
Molecular Probes Handbook Phycobiliproteins—Section 6.4 antibody labeling, multicolor flow cytometry, tandem conjugates
Molecular Probes Handbook Microspheres—Section 6.5 flow cytometer calibration, fluorescence microscopy/fluorescence imaging, microscope calibration, particles
Molecular Probes Handbook Flow cytometry reference standards—Section 23.2 flow cytometer
Molecular Probes Handbook Using the Fluorescence SpectraViewer—Note 23.1 flow cytometry, fluorescence, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins
Molecular Probes Handbook MHC tetramer technology—Note 7.2 antibodies, flow cytometry, immunology, Major Histocompatibility Complexes, T cells
Molecular Probes Handbook Probes for mitochondria—Section 12.2 antibodies, cell structure-mitochondria, CellLight, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes
Molecular Probes Handbook Probes for lysosomes, peroxisomes and yeast vacuoles—Section 12.3 antibodies, cell structure-lysosomes, CellLight, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes
Molecular Probes Handbook Probes for the nucleus—Section 12.5 antibodies, cell structure-nucleus, CellLight, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, membrane permeability
Molecular Probes Handbook Probes useful at near-neutral pH—Section 20.2 BCECF, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, pH detection, pH indicators, SNARF
Molecular Probes Handbook Probes useful at acidic pH—Section 20.3 flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, live-cell imaging, LysoSensor, pH detection, pH indicators, pHrodo
Molecular Probes Handbook pH indicator conjugates—Section 20.4 flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, live-cell imaging, LysoSensor, pH detection, pH indicators, pHrodo
Molecular Probes Handbook Overview of pH indicators—Section 20.1 fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, live-cell imaging, LysoSensor, pH detection, pH indicators, pHrodo
Molecular Probes Handbook Nucleic acid stains—Section 8.1 cell cycle, cell structure-nucleus, cell structure-plasma membrane, cell viability, DAPI, DNA binding dyes, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, membrane permeability, RNA binding dyes, SYTO, SYTOX
Molecular Probes Handbook Tracers for membrane labeling—Section 14.4 cell structure-plasma membrane, cell tracking, flow cytometry, fluorescence microscopy/fluorescence imaging, live-cell imaging
Molecular Probes Handbook Microspheres and Qdot Nanocrystals for tracing—Section 14.6 blood flow, FluoSpheres, neuronal tracing, particles, Qdot, Qtracker, quantum dots
Molecular Probes Handbook Viability and cytotoxicity assay reagents—Section 15.2 cell health, cell viability and function, fluorescence microscopy/fluorescence imaging, microplate reader
Molecular Probes Handbook Viability and cytotoxicity assay kits for diverse cell types—Section 15.3 cell health, cell viability and function, fluorescence microscopy/fluorescence imaging, microplate reader
Molecular Probes Handbook Assays for apoptosis and autophagy—Section 15.5 apoptosis, autophagy, flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging
Molecular Probes Handbook Zenon Technology: Versatile reagents for immunolabeling—Section 7.3 antibody labeling, flow cytometry, fluorescence microscopy/fluorescence imaging, secondary detection, Zenon
Pathways Adaptive immunity pathways immunology
Pathways Innate immunity pathways immunology
Pathways  Inflammatory response pathway  
Pathways Interferon (IFN) cell signaling pathway  
Pathways  T-cell receptor (TCR) cell signaling pathway  
Pathways Toll-like receptor (TLR) cell signaling pathway  
Protocols Annexin V staining protocol for flow cytometry (Invitrogen eBioscience reagents) apoptosis, flow cytometry
Protocols BrdU Staining Protocol for Flow Cytometry (Invitrogen eBioscience reagents) cell proliferation, flow cytometry
Protocols cell Preparation for flow cytometry protocols (Invitrogen eBioscience reagents) flow cytometry, sample preparation
Protocols CellTrace CFSE cell proliferation Kit protocol cell proliferation, flow cytometry
Protocols CellTrace Far Red cell proliferation Kit protocol cell proliferation, flow cytometry
Protocols CellTrace Violet cell proliferation Kit protocol cell proliferation, flow cytometry
Protocols LIVE/DEAD Fixable Dead cell stains protocol flow cytometry, viability
Protocols LIVE/DEAD Sperm Viability Kit flow cytometry protocol flow cytometry, viability
Protocols LIVE/DEAD Violet Viability/Vitality Kit protocol flow cytometry, viability
Protocols OneComp and UltraComp Compensation Beads protocols for flow cytometry (Invitrogen eBioscience reagents) compensation, flow cytometry
Protocols Pharmacological Induction of Apoptosis with Camptothecin (Invitrogen eBioscience reagents) apoptosis, flow cytometry
Protocols Red blood cell lysis protocols using eBioscience lysis buffers (Invitrogen eBioscience reagents) flow cytometry, sample preparation
Protocols Staining cell surface targets for flow cytometry (Invitrogen eBioscience reagents) extracellular staining, flow cytometry, immunophenotyping
Protocols Staining cells with eFluor proliferation dyes for flow cytometry (Invitrogen eBioscience reagents) cell proliferation, flow cytometry
Protocols Staining intracellular antigens for flow cytometry (Invitrogen eBioscience reagents) flow cytometry, immunophenotyping, intracellular staining
Protocols SYTOX Dead Cell Stains protocol cell viability, flow cytometry
Protocols  Super Bright staining buffer protocol  antibody conjugates, flow cytometry, immunophenotyping
Protocols Viability staining protocol for flow cytometry (Invitrogen eBioscience reagents) cell viability, flow cytometry
Scientific poster (2018)  Induced pluripotent stem cell sorting, culture and differentiation to desired cell lineage  Attune/Attune NxT, cell counting, cell sorting, Countess, EVOS, fluorescence activated cell sorting, iSort Automated Cell Sorter, imaging microscopy, stem cell research
Scientific poster (2018)  Modular, defined assembly of antibody-based constructs using click chemistry  Alexa Fluor, antibody labeling, Attune/Attune NxT, CellInsight, drug discovery, drug research, high content analysis (HCA), SiteClick, spheroids
Scientific poster (2018)  Hypoxia measurements in live and fixed cells using fluorescence microscopy, high-content imaging and flow cytometry  Attune/Attune NxT, brightfield microscopy, CellInsight, EVOS, high content analysis (HCA), hypoxia, spheroids
Scientific poster (2018)  Evaluation of multiple techniques for PMT optimization  Attune/Attune NxT Flow Cytometer, flow cytometer calibration, photomultiplier settings
Scientific poster (2018)  PUBLISH OR PERISH? Initiating, maintaining, and growing a publication history within an SRL role  flow cytometry, career advancement, shared lab resources
Scientific poster (2018)  Prospective cytometric study evaluating the alkaline phosphatase activity as a potential functional biomarker of primitive malignant cells in acute leukemia  antibodies, Attune/Attune NxT, cancer, cell function, flow cytometry, immunophenotyping, stem cell research
Scientific poster (2018)  Synergistic effect of curcumin in combination with proapoptotic drugs in human cell models for chronic (CLL) lymphocytic leukaemia  Attune/Attune NxT Flow Cytometer, cell cycle, cancer, drug development, drug research, flow cytometry
Scientific poster (2018)  Differently cell doublet profiles: comparison of light scattering with fluorescence measurement techniques  Attune/Attune NxT Flow Cytometer, cell counting, cell cycle, cell viability, flow cytometry, single cell discrimination
Scientific poster (2018)  Differential expression of PD-L1 on myeloid derived suppressor cells as a potential screening approach for cancer immunotherapy in multiple myeloma  Attune/Attune NxT, cancer, drug research, , immunology, immuno-oncology, multicolor flow cytometry, translational research solutions
Scientific poster (2018)  Maintaining even temperature distribution over chilled samples in 96-well plates and micro-centrifuge tubes  academic research solutions, microplates, quality control, temperature control
Scientific poster (2018)  A comparison of GFP BrightComp eBeads Compensation Bead Kit performance against multiple GFP variants  Attune/Attune NxT, beads, flow cytometry compensation, fluorescent protein detection,
Scientific poster (2018)  Fluorescent hypoxia sensor for flow cytometry  apoptosis, Attune/Attune NxT, brightfield microscopy, EVOS, flow cytometry, hypoxia, immunology
Scientific Poster (2017)  T-lymphocyte immunophenotyping: 14-color flow cytometry panel design using the Attune NxT Flow Cytometer and Super Bright fluorescent dyes  antibodies, Attune NxT, fluorescent dyes, immunophenotyping, multicolor flow cytometry, particles, Super Bright
Scientific Poster (2016)  A microsphere-based universal compensation approach Attune NxT, Flow cytometer calibration, flow cytometry sample preparation
Scientific Poster (2016)  A novel non-cytotoxic fluorescent dye for cell proliferation analysis in flow cytometry  Attune NxT, cell health, cell proliferation, CellTrace, EVOS, flow cytometry, fluorescence microscopy/fluorescence imaging
Scientific Poster (2016)  An objective test to qualify plate samplers for complex flow cytometric assays  Attune NxT, Autosampler, flow cytometer
Scientific Poster (2016)  Cell mediated cytotoxicity in “untouched” whole blood  Attune NxT, cell concentration, cell cycle, flow cytometry assays, viability, whole blood
Scientific Poster (2016)  No-lyse no-wash new strategy for CD34+ for absolute cell counting without beads  antibodies, Attune NxT, cell counting, compensation, flow cytometry assays , fluorescent dyes
Scientific Poster (2015)  A no lyse, no-wash approach to characterizing phagocyte phenotype and function in whole human blood on the Attune NxT Flow Cytometer  Attune NxT, cell cycle, flow cytometer, flow cytometry assays, immunophenotyping, no lyse, phagocytosis, whole blood
Scientific poster (2014) Copper-safe Click-iT Plus EdU proliferation assay: Improved compatibility with simultaneous phycobiliprotein and fluorescent protein detection cell proliferation, Click-iT, flow cytometry, immunophenotyping
Scientific poster (2014) Flow cytometry multiplexing used to analyze metabolic activity and assess pharmaceutical compound toxicity as a high-throughput screening tool Attune NxT, drug discovery, flow cytometer, flow cytometry assay
Scientific Poster (2014)  Click-iT EdU Plus for flow cytometry: enhanced compatibility for multiplexing  Attune NxT, cell proliferation, Click-iT, multicolor flow cytometry
Scientific poster (2013)  Cell-based measurements of oxidative stress with a new near infrared emitting probe for reactive oxygen species: Applications for multiplexed evaluation of cell health  apoptosis, ArrayScan, caspase substrates, cell health, cells, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, high content analysis, microplate reader, oxidative stress
Scientific Poster (2013)  Cells coming to life: Tools for visualizing immune response  endocytosis/phagocytosis, flow cytometry, fluorescence microscopy/fluorescence imaging, live-cell imaging, pH detection, T cell stimulation
Scientific poster (2013)  Improved click chemistry demonstrating EdU cell proliferation with GFP-expressing cells and R-PE-based immunophenotyping  Alexa Fluor, cell cycle, cell proliferation, Click-iT, flow cytometry assays, fluorescent dyes, fluorescent proteins, immunophenotyping
Scientific poster (2013)  Monitor caspase 3/7 activity without cell fixation: A novel apoptosis reagent Alexa Fluor, apoptosis, Attune NxT, caspase substrates, cells, fluorescent dyes, multicolor flow cytometry
Scientific poster (2013)  Reactive oxygen probes—A broad range of colors with easier labeling and compatibility with fixation: Novel CellROX reagents cells, fluorescent dyes, multicolor flow cytometry, oxidative stress
Scientific poster (2013)  Reactive oxygen probes—A broad range of colors with easier labeling: Novel CellROX reagents cells, fluorescent dyes, multicolor flow cytometry, oxidative stress, viability
Scientific poster (2011)  Rare event analysis: Detection of mouse plasmacytoid dendritic cells and human circulating endothelial cells  Alexa Fluor, endothelial cells, flow cytometry, fluorescent dyes, rare event detection
Scientific poster (2011)  The next step in the analysis of lymphocyte proliferation Alexa Fluor, antibodies, Attune NxT, cell proliferation, CellTrace, flow cytometry assays, fluorescent dyes, live-cell imaging, violet laser-excited reagents
Scientific poster (2010)  A comparison of three techniques to induce efficient ex vivo T cell expansion  Alexa Fluor, antibodies, cell proliferation, CellTrace, flow cytometry, fluorescent dyes, immunology, magnetic beads, multicolor flow cytometry, viability
Scientific poster (2010)  Analysis of proliferation dynamics in primary and immortalized human cells  Alexa Fluor, cancer, cell proliferation, CellTrace, circulatory system, Click-iT, flow cytometry, fluorescent dyes, fluorescent proteins, nucleic acid quantitation
Scientific poster (2010)  Dual pulse labeling using EdU and BrdU to monitor alterations in cellular proliferation by fluorescence microscopy and flow cytometry  Alexa Fluor, cell cycle, cell proliferation, cells, Click-iT, drug discovery, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes
Scientific poster (2010)  Functional characterization of a novel fluorescent dye for proliferation analysis  Alexa Fluor, cell proliferation, cells, CellTrace, flow cytometry, fluorescent dyes, violet laser-excited reagents
Scientific poster (2010)  Non-cytotoxic near-IR DNA stain for cell cycle analysis in living cells  cell cycle, flow cytometry, fluorescent dyes, fluorescent proteins, live-cell imaging, nucleic acid labeling, viability
Scientific poster (2010)  Proliferative and phenotypic characterization of human mesenchymal stem cells by flow cytometry and imaging Alexa Fluor, cell proliferation, cells, Click-iT, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, immunofluorescence (IF), immunophenotyping, stem cell research, violet laser-excited reagents
Scientific poster (2010)  Simultaneous analysis of cell death mechanisms and oxidative stress using Molecular Probes next generation reagents for imaging and flow cytometry  apoptosis, autophagy, caspase substrates, cells, flow cytometry/flow cytometer, fluorescence microscopy/fluorescence imaging, fluorescent dyes, live-cell imaging, oxidative stress
Scientific poster (2010)  Use of surface-modified microspheres for setting compensation in multicolor flow cytometry  antibodies, cells, flow cytometry compensation, fluorescent dyes, multicolor flow cytometry, particles, violet laser-excited reagents
Scientific poster (2009)  A comparative study of 25 different CD4 conjugates  Alexa Fluor, antibodies, fluorescent dyes, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot
Scientific poster (2009)  A novel xeno-free and serum-free culture medium for the expansion of human mesenchymal stem cells  cell culture, cell expansion, flow cytometry, fluorescent dyes, gene expression, immunophenotyping, stem cell research
Scientific poster (2009)  A simple, fast and quantitative single-step dead-cell indicator for flow cytometry  apoptosis, fluorescent dyes, multicolor flow cytometry, nucleic acid quantitation, viability
Scientific poster (2009)  Dead cell stains in flow cytometry: A comprehensive analysis  apoptosis, cells, flow cytometry, fluorescent dyes, immunophenotyping, viability
Scientific poster (2009)  Dual pulse labeling of S-phase population using click chemistry to measure changes in cell proliferation  Alexa Fluor, cancer, cell cycle, cell proliferation, cells, Click-iT, flow cytometry, fluorescent dyes
Scientific poster (2009)  Dual pulse labeling using a new thymidine analog 5-ethynyl-2’-deoxyuridine (EdU) to detect alterations in S-phase progression by fluorescence microscopy and flow cytometry  Alexa Fluor, cell cycle, cell proliferation, cells, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes
Scientific poster (2009)  Proliferative and phenotypic characterization of human mesenchymal stem cells by flow cytometry  Alexa Fluor, cell proliferation, cells, Click-iT, flow cytometry, fluorescent dyes, fluorescent proteins, immunophenotyping, nucleic acid quantitation, stem cell research, violet laser-excited reagents
Scientific poster (2008)  A new 405 nm excitable fluorescent probe of intracellular thiols for imaging and flow cytometric assays  ArrayScan, cells, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, glutathione, microplate reader, violet laser-excited reagents
Scientific poster (2008)  Characterization of DNA content, cyclin B1 and phosphorylated histone H3 with direct S-phase using EDU incorporation in multiparameter testing of cell lines with cell cycle blocking agents  Alexa Fluor, antibodies, apoptosis, ArrayScan, cell cycle, cell proliferation, cells, Click-iT, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes
Scientific poster (2008)  Detection of DNA synthesis by automated microscopy and image analysis: Comparison of BRDU method and a new click chemistry-based EDU method  Alexa Fluor, antibodies, ArrayScan, cell cycle, cell proliferation, cells, Click-it, flow cytometry, fluorescent dyes, microplate reader, nucleic acid labeling
Scientific poster (2008)  Detection of S-phase cell cycle progression using 5-ethynyl-2'-deoxyuridine incorporation with click chemistry  Alexa Fluor, antibodies, ArrayScan, cell cycle, cells, Click-iT, flow cytometry, fluorescent dyes, microplate reader, nucleic acid labeling, nucleic acid quantitation
Scientific poster (2008)  Effects of the thymidine analogues EdU and BrdU on cell viability and cycle progression  Alexa Fluor, cell cycle, cell proliferation, cells, Click-iT, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, viability
Scientific poster (2008)  Zenon labeling technology facilitates detection of intra-cellular phosphoprotein determinants  Alexa Fluor, antibodies, cell signaling, cells, flow cytometry, fluorescent dyes, protein labeling, rare cell detection, Zenon
Scientific poster (2007)  A new approach for the detection of intracellular glutathione by fluorescence microscopy and flow cytometry  ArrayScan, cells, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, glutathione, live-cell imaging, microplate reader, viability, violet laser-excited reagents
Scientific poster (2007)  Evaluation of click chemistry-based alternative to BrdU antibody labeling in tissue and cultured cells using fluorescence microscopy and flow cytometry  antibodies, ArrayScan, cell cycle, cell proliferation, cells, Click-iT, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, microplate reader, nucleic acid labeling
Scientific poster (2006)  Novel violet-excited reagents for detection of viability and vitality   Alexa Fluor, cell health, CellTrace, flow cytometry, fluorescent dyes, viability, violet laser-excited reagents
Selection guide Antibody fixation considerations sample preparation
Selection guide Human antibody cross reactivity chart antibody cross-reactivity, flow cytometry
Selection guide Mouse antibody cross reactivity chart antibody cross-reactivity, flow cytometry
Selection guide Cytokine bioassays guide cytokines, flow cytometry, immunophenotyping, intracellular staining
Selection guide Intracellular staining guide (for mouse & human cytokines and non-cytokine proteins) cytokines, flow cytometry, intracellular staining
Selection guide Human CD & other cellular antigens flow cytometry, immunology, immunophenotyping, extracellular staining
Selection guide Intracellular staining buffer selection guide flow cytometry, immunophenotyping, intracellular staining
Selection guide Mouse CD & other cellular antigens flow cytometry, immunology, immunophenotyping, extracellular staining
Selection guide Phospho flow cytometry antibody clone buffer selection guide flow cytometry, immunophenotyping, intracellular staining, phosphoflow cytometry