| Most common method Antibody binding |
You have a biotinylated antibody towards the antigen Biotin binding |
You have cell line with a recombinant (fusion tagged) target protein Recombinant protein binding |
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|---|---|---|---|
| Starting sample | Lysate from any cell/ bacteria/yeast, or body fluids | Lysate from any cell/ bacteria/yeast, or body fluids | Cell lines |
| Requires additional target antibody | Yes | Yes | No, beads are directly targeting the tagged proteins |
| Requires species specific antibodies | Yes | No | N/A |
| Antibody co-eluted off the beads | Yes/No (product specific) | Yes* | N/A |
| Notes |
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Everyone starting out in fluorescence imaging has questions. Learn the fundamentals of fluorescence microscopy, including sample and media considerations, sample preparation, and beginner’s protocols to help you get started. We hope you will benefit from the information in Molecular Probes School of Fluorescence.
Step 1: Search for the target species of interest (e.g., “anti-mouse” or “goat anti-rabbit” or “alexa fluor 488”).
Step 2: Narrow results by host species, conjugate, application, and other criteria using side filters.
| By host species | By antibody isotype | By conjugate type |
|---|---|---|
| Goat | IgA | Unconjugated |
| Hamster | IgE | Alexa Fluor dyes |
| Human | IgG | Biotin conjugates |
| Mouse | IgG1 | Classic dyes (e.g. FITC, TRITC) |
| Rabbit | IgG2a | Pacific dyes |
| Rat | IgG2b | PE and APC |
| IgG3 | PerCP | |
| IgM | Qdot probe | |
| Multi | Tandems (e.g. PE-Alexa Fluor 647) | |
| TRI-COLOR |
