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 Most common method
Antibody binding
You have a biotinylated antibody towards the antigen
Biotin binding
You have cell line with a recombinant (fusion tagged) target protein
Recombinant protein binding
Starting sampleLysate from any cell/ bacteria/yeast, or body fluidsLysate from any cell/ bacteria/yeast, or body fluidsCell lines
Requires additional target antibodyYesYesNo, beads are directly targeting the tagged proteins
Requires species specific antibodiesYesNoN/A
Antibody co-eluted off the beadsYes/No (product specific)Yes*N/A
Notes
  • High specificity for different antibodies.
  • Optimized IP protocols and ready-to-go-kits
  • Mass spectrometry compatible products
  • Binds any biotinylated antibody, thus very flexible use
  • Doesn’t require an antibody to isolate the target.
  • Same tag can be used on many different proteins
*Crosslinking can be performed to avoid co-elution of the antibody, but this can decrease the yield of the target antigen.
Bulk Quantities and Custom Products

Partner with us to scale-up your process or product development pipeline with bulk quantities of high-quality immunoassay reagents and biochemicals. We offer a variety of compounds, devices, and resins for your large-scale applications.

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Molecular Probes School of Fluorescence

Molecular Probes School of Fluorescence

Everyone starting out in fluorescence imaging has questions. Learn the fundamentals of fluorescence microscopy, including sample and media considerations, sample preparation, and beginner’s protocols to help you get started. We hope you will benefit from the information in Molecular Probes School of Fluorescence.

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How to find a secondary antibody

Step 1: Search for the target species of interest (e.g., “anti-mouse” or “goat anti-rabbit” or “alexa fluor 488”).
Step 2: Narrow results by host species, conjugate, application, and other criteria using side filters.

Popular products

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LPC1171M_210x100f
LPC1171M_210x100b
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Popular products

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By host speciesBy antibody isotypeBy conjugate type
GoatIgAUnconjugated
HamsterIgEAlexa Fluor dyes
HumanIgGBiotin conjugates
MouseIgG1Classic dyes (e.g. FITC, TRITC)
RabbitIgG2aPacific dyes
RatIgG2bPE and APC
 IgG3PerCP
 IgMQdot probe
 MultiTandems (e.g. PE-Alexa Fluor 647)
  TRI-COLOR

Search for primary or secondary antibodies