flourecence-imaging
photobleach-banner-362x140
Alexa fluor secondary antibodies
western-collection-banner-362x140
ibind-banner-362x140
WE226266-Template_DarkPhoto-090215-362x140-SP_BXX
Protein transfer technical handbook
attune-banner-362x140
Custom fluorescent antibody and protein labeling service
Free white paper
Free antibodies for data
Western Freedom
Western Wow
Western Done
Free white paper
Guide to antibody purification
Custom Antibodies Services
40000-antibodies
EVOS imaging systems
Alexa Fluor® Labeling Kits
Protein quantitation immunoassays

Featured custom antibody categories

ELISA and additional screening and method validation procedures for custom antibodies.

Thermo Fisher Scientific online project management tool for Pierce Custom Antibody Production Services enables you to easily track and efficiently manage your antibody development projects.

Choose the custom antibody services based on specific assay applications and overall price.

Custom antibody services for production and purification of post-translational modification-specific antibodies.

174

The Thermo Scientific Antigen Profiler is a bioinformatics protein sequence analysis tool and custom peptide design algorithm for designing and creating superior peptide antigens. The platform incorporates an exclusive set of powerful bioinformatics algorithms for analyzing and designing peptide and protein antigens to ensure high-titer custom production of highly-specific antibodies. The analysis is offered as a service for researchers who wish to design and synthesize effective peptide antigens for custom antibody development using our comprehensive custom antibody production service.

Assay Development Technical Handbook

The revised Assay Development Technical Handbook is an essential resource for any laboratory using enzyme-linked immunosorbent assay (ELISA) and related plate-based assay methods. The handbook describes the essential techniques and tools for designing and optimizing ELISA Assays. Featured products include coated microplates, standards, blockers, buffers, probe-labeling reagents, secondary antibodies and detection substrates.

Contents include: Introduction to ELISA, Selecting an ELISA Plate, Thermo Scientific Pierce Microplates, Thermo Scientific Pierce Coated Microplates, Blocking and Washing, Blocking and Washing Reagents, Detection Probes, Antibody Labeling, Choosing a Substrate, Bulk and Custom Offerings, and Recommended Reading.

Up to 30% off Alexa Fluor® secondary antibodies
photobleach-banner-362x140

Cell and Protein Isolation Technical Handbook

Learn how to optimize protein extraction from cells and tissue for better yield and improved downstream compatibility using Thermo Scientific™ Pierce™ Protein Biology Products.

  • Extract total protein quickly and efficiently
  • Obtain subcellular fractions with minimal cross- contamination
  • Enhance lysis and immunoassay techniques with highly purified, prediluted detergents
  • Remove interfering detergents rapidly and effectively
High-Performance Dialysis Brochure

Dialysis is a separation technique that gained popularity in life science laboratories during the 1950s. Research papers of that era described dialysis as a new, cutting-edge tool that scientists could use to unravel complex mixtures of biomacromolecules. Many of the dialysis theories established at that time are the cornerstones for contemporary products featured in this brochure. There are, however, two major differences between the dialysis tools of yesterday and today—preparation time and the amount of sample loss due to leaks. Early laboratory dialysis methods involved dedicating a significant amount of time to membrane preparation; Thermo Scientific Pierce Dialysis Products are essentially ready to use and resist sample leakage.

Zeba Desalting Handbook

Thermo Scientific Zeba Resin technology provides consistent performance and peace of mind. The unique, proprietary Zeba Resin enables better protein recovery, even for samples with low protein concentrations.

Protein Assay Technical Handbook

The 40-page handbook reviews the principle of four major protein assay chemistries and contains an updated substance compatibility list. In addition, reaction schemes, protein-to-protein variation data, protocol schematics and a quick technical summary table accompany each assay methodology. The table includes the working range of the assay, characteristics and advantages, applications, disadvantages, and interfering substances. Useful references are provided with each assay.

Protein Gel Electrophoresis Technical Handbook

This 88-page handbook provides detailed description about all aspects of protein electrophoresis from sample and gel preparation to choice of molecular weight markers. In addition, it contains extensive information about our portfolio of high-quality protein electrophoresis products including gels, stains, molecular weight markers, running buffers, and blotting products for your experiments.

Request download

Avidin-Biotin Technical Handbook

Our 48-page Avidin-Biotin Technical Handbook brings together everything needed to biotinylate, purify or detect proteins. Featured products include cell-surface protein biotinylation and purification kits, antibody labeling and new photo-reactive biotinylation reagents. This handbook includes dozens of references along with protocols, troubleshooting tips, selection guides and a complete listing of available tools.

Western Blotting Handbook and Troubleshooting Guide

The "Thermo Scientific Pierce Western Blotting Handbook and Troubleshooting Guide" (2014) details each step of the Western blotting process with technical information and products for transfer, blocking, washing, antibodies, substrates, film and stripping buffer, including Pierce Fast Western products. You will want to keep this booklet close at hand because it also includes protocols, references and a troubleshooting guide.

Protein Interactions Handbook

Our 72-page Protein Interaction Technical Handbook provides protocols and technical and product information to help maximize results for Protein Interaction studies. The handbook provides background, helpful hints and troubleshooting advice for immunoprecipitation and co-immunoprecipitation assays, pull-down assays, Far-Western blotting and crosslinking. The handbook also features an expanded section on method to study protein:nucleic acid interactions, including ChIP, EMSA and RNA EMSA. The handbook is an essential resource for any laboratory studying Protein Interactions.

Contents include: Introduction to Protein Interactions, Co-Immunoprecipitation, Pull-Down Assays, Crosslinking Reagents, Label Transfer, Far-Western Blotting, Protein Interaction Mapping, Yeast Two-hybrid Reporter Assay, Electrophoretic Mobility Shift Assays [EMSA], Chromatin Immunoprecipitation Assays (ChIP), Protein:Nucleic Acid Conjugates, and more.

Mass Spec Analysis Technical Handbooks

All the tools you need to optimize your mass spec experiments
Download our new products brochure and any of our technical handbooks to help you improve your mass spectrometry experiments with Thermo Scientific™ Pierce™ Protein Biology Products.

  • Handbook 1: Thermo Scientific 2015 New Product Guide for Mass Spectrometry
  • Handbook 2: Thermo Scientific Pierce Controls and Standards for Mass Spectrometry
  • Handbook 3: Thermo Scientific Pierce Products for Mass Spectrometry Sample Preparation
  • Handbook 4: Thermo Scientific Pierce Reagents for Quantitative Proteomics

Quantitative Proteomics Handbook

Our Quantitative Proteomics Handbook provides an overview of protein quantitation tools and reagents for discovery and targeted proteomics.

Bioconjugate Techniques, 3rd Edition

Bioconjugate Techniques, 3rd Edition (2013) by Greg T. Hermanson is a major update to a book that is widely recognized as the definitive reference guide to the field of bioconjugation.

Bioconjugate Techniques is a complete textbook and protocols-manual for life scientists wishing to learn and master the biomolecular crosslinking, labeling and immobilization techniques that form the basis of many laboratory applications. The book is also an exhaustive and robust reference for researchers looking to develop novel conjugation strategies for entirely new applications. It also contains an extensive introduction to the field of bioconjugation that covers all of the major applications of the technology used in diverse scientific disciplines as well as containing tips for designing the optimal bioconjugate for any purpose.

Antibody production and purification guide

The updated Antibody Production and Purification Technical Handbook is an essential resource for any laboratory working with antibodies. The handbook provides an overview of antibody structure and types, as well as technical information on the procedures, reagents and tools used to produce, purify, fragment and label antibodies.

Crosslinking Reagents Technical Handbook

This 45-page guide is of value to the novice as well as those who have previous experience with crosslinking reagents. It begins with a basic discussion on crosslinking and the reagents that are used. The guide also contains a discussion on various applications where crosslinking has been applied, including the powerful label-transfer technique for identifying or confirming protein interactions. Crosslinking chemistry is addressed in an easy-to-follow format designed to convey the important information you need without getting lost in details. Each Pierce crosslinking reagent is shown along with it's structure, molecular weight, spacer arm length and chemical reactivity. The handbook concludes with a list of excellent references on cross-linker use and a glossary of common crosslinking terms.

Protein Purification Technical Handbook

The revised 80-page handbook provides technical and product information to help maximize results for protein purification procedures. The handbook provides background, helpful hints and troubleshooting advice for covalent coupling of affinity ligands to chromatography supports, avidin:biotin-binding, affinity purification of antibodies, immunoprecipitation and co-immunoprecipitation assays, affinity procedures for contaminant removal, and FPLC purification methods.

ProteinPurTechHndbk150x200

Featured categories

Antibody Pair Kits

Matched, pre-titered, and fully optimized capture (coating) and detection antibodies allow you to build your own immunoassay.

Primary Antibodies

Search for primary antibodies against a wide range of markers, including signaling proteins, cell and organelle markers, cell junctions CD markers, and more.

Luminex® Assays

Bead-based technology enables the analysis of multiple targets in a single sample from a wide range of biological sources. Check our custom Luminex® assay panel.

Secondary Antibodies

Browse our secondary antibodies, with conjugates that include Alexa Fluor® and classic fluorescent dyes, HRP and AP, and more from a wide range of hosts.

Resources

Molecular Biology Resource Library

Webinars, videos, articles, and selection guides

Molecular Biology Web Tools

Tools for primer analysis, reaction setup, biochemical conversions, and calculators