Cell Isolation With Dynabeads and MagniSort Technology

Gentle, tube-based magnetic separation with Invitrogen Dynabeads or MagniSort beads are technologies of choice when you want to isolate high yields of pure, viable and functional cells. Magnetic separation helps ensure that the isolated cells are not affected by passage through a dense column and that the cells’ native characteristics are preserved.

Human cell isolation productsMouse cell isolation products

Positive cell isolation

In positive cell isolation, Dynabeads bind the target cells and the bead/cell complexes are pulled to the magnet. The supernatant is discarded, leaving only your pure target cells.

Bead-free cells

Directly after cell separation, the beads can easily be released and removed using the Dynabeads FlowComp technology.

Negative cell isolation

In negative cell isolation, Dynabeads are used to deplete all the unwanted cells using the supplied antibody mix.  Your bead-free and untouched target cells are left in the sample, and can be directly analyzed in a flow cytometer and used in any functional assay.

To refine for a particular cell type, you can of course add extra antibodies to the antibody mix. With the secondary antibody–coated Dynabeads sheep anti-rabbit IgG and sheep anti-mouse IgG you can create your own negative isolation kit for isolation of any cells from any species.

Depletion

In depletion, cell-specific Dynabeads are used to remove specific unwanted cell types from a mixed cell sample with >99% efficiency. Recover the depleted supernatant—containing all your cells of interest—for downstream assays.

Magnetic beads for other cell isolation

You can also use the positively targeted and bead-bound cells for downstream molecular applications that do not require release of cells.

Automate your cell isolation on the Thermo Scientific KingFisher family of instruments. KingFisher instruments have the ability to eliminate any cross contamination, have reproducible results and cut your hands on time down to 40 min. Set up plates, press start and walk away.

See how the KingFisher instrument works with cells and download the cell isolation protocol for your KingFisher today.  

KingFisher Instrument

Positive cell isolation

In positive cell isolation, Dynabeads bind the target cells and the bead/cell complexes are pulled to the magnet. The supernatant is discarded, leaving only your pure target cells.

Bead-free cells

Directly after cell separation, the beads can easily be released and removed using the Dynabeads FlowComp technology.

Negative cell isolation

In negative cell isolation, Dynabeads are used to deplete all the unwanted cells using the supplied antibody mix.  Your bead-free and untouched target cells are left in the sample, and can be directly analyzed in a flow cytometer and used in any functional assay.

To refine for a particular cell type, you can of course add extra antibodies to the antibody mix. With the secondary antibody–coated Dynabeads sheep anti-rabbit IgG and sheep anti-mouse IgG you can create your own negative isolation kit for isolation of any cells from any species.

Depletion

In depletion, cell-specific Dynabeads are used to remove specific unwanted cell types from a mixed cell sample with >99% efficiency. Recover the depleted supernatant—containing all your cells of interest—for downstream assays.

Magnetic beads for other cell isolation

You can also use the positively targeted and bead-bound cells for downstream molecular applications that do not require release of cells.

Automate your cell isolation on the Thermo Scientific KingFisher family of instruments. KingFisher instruments have the ability to eliminate any cross contamination, have reproducible results and cut your hands on time down to 40 min. Set up plates, press start and walk away.

See how the KingFisher instrument works with cells and download the cell isolation protocol for your KingFisher today.  

KingFisher Instrument

MagniSort cell isolation products

Starting with T cell-specific cocktails, use the MagniSort platform to obtain enriched T cells simpler, faster and with significant cost-savings compared to traditional column-based separation methods.

Learn more about the MagniSort platform