Bacterial RNA Extraction
Unlike eukaryotic cells, yeast and many bacterial strains are protected by cell walls that can be difficult to disrupt. As a first step, effective cell lysis (typically via mechanical disruption or enzymatic pretreatment) is required prior to any RNA isolation procedure.
A range of products developed by the Ambion® RNA experts will help you isolate high yields of pure, intact RNA from bacteria and yeast.
Which Bacterial RNA Extraction Kit is Right for You?
|No lysis procedures||Fast & easy, 20 min||HTP, 45 min||Ideal for hard-to-lyse gram + bacteria|
|Order now||Order now||Order now||Order now|
|TRIzol® Max™ Bacterial RNA Isolation Kit w/TRIzol® & Max Bacterial Enhancement Reagent||PureLink® RNA Mini Kit||MagMAX™ Total Nucleic Acid Isolation Kit||RiboPure™-Bacteria Kit|
|Includes specialized beads for cell lysis||No||No||Zirconia beads||Zirconia beads|
|Isolation method||High purity organic extraction (requires alcohol precipitation)||Fast, convenient silica column||Scalable, flexible format with magnetic beads||Highest purity & convenience; includes organic extraction & silica column (no precipitation required)|
|Prep time||1 hour||20 minutes||45 minutes||1 hour|
|Amount of starting material||Up to 1 x 108 cells||Up to 1 x 109 cells||Up to 0.3 g solid samples or 175 µl liquid samples||Up to 109 cells|
|High throughput compatible||✓|
|Kit size||100 preps||50 preps||100 preps||50 preps|
|Price per prep||$4.43||$4.50||$5.24||$7.58|