Tissue RNA Extraction
Isolating intact total RNA from tissue samples varies in difficulty with the physical and biochemical nature of that tissue. While many methods will work equally well for tissues such as liver, fibrous tissues (e.g. heart), fatty tissues (e.tg. brain), and nuclease-rich tissues (e.g. spleen) are more challenging.
A range of products developed by our RNA experts will help you isolate high yields of pure, intact RNA from almost any type of tissue.
Which tissue RNA extraction kit is right for you?
|Gold standard for highly pure, intact RNA||Simple, reliable, rapid method||Consistent isolation from micro-sized samples (LCM & fine needle aspirates)||Highest purity TRIzol® + column purification, no precipitation required||HTP format,capable of recovering RNA of all sizes (inlcuding microRNAs)|
|Order now||Order now||Order now||Order now||Order now|
|TRIzol® Reagent||PureLink™ RNA Mini Kit||RNAqueous® - Micro Kit||TRIzol® Plus RNA Purification System||MagMAX mirVana Total RNA Isolation|
|Top Seller||Most cited, Top seller|
|Isolation method||High purity organic extraction (requires alcohol precipitation)||Fast, convenient silica column||Fast, convenient low elution silica column||Highest purity and convenience; includes both organic extraction and silica column (no precipitation required)||Scalable, flexible format with magnetic beads|
|Prep time||1 hour||20 minutes||15 minutes||1 hour||<1 hour|
|Amount of starting material||Up to 100 mg||10 mg to 100 mg||10 cells to 10 mg||Up to 100 mg||Up to 50 mg|
|High throughput compatible||✓|
|Prep size||100 preps||50 preps||50 preps||50 preps||96 preps|
RNA Yields From Animal Tissues
RNA content can vary widely between tissues, cell types, physiological states, etc. This chart provides general guidelines for estimating RNA yields from a variety of cells and tissues.
Table 1. Typical total RNA yields from 1 mg of various tissues.
Preserve and Stabilize Your RNA
RNAlater® Solution is widely used for the preservation of RNA in intact animal tissues, organs, and cells, and bacteria. It eliminates the need to immediately process samples for RNA isolation or to freeze them in liquid nitrogen for later processing.