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See how KingFisher systems can support a broad range of applications. Want to see how it works in your lab?

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DNA for downstream analysis

Extract high-quality DNA from a range of sample types that is compatible with sequencing or array platforms. Choose from one of two protocols for either large or small volume inputs, without normalization.

Sample workflow

Sample workflow

Recommended kits

  Applied Biosystems MagMAX DNA Multi-Sample Ultra 2.0 Kit
Applied Biosystems MagMAX DNA Multi-Sample Ultra 2.0 Kit
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KingFisher Ready DNA Ultra 2.0 Kit
KingFisher Ready DNA Ultra 2.0 Kit
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Sample type Multiple, including whole blood, swabs, tissue, and more Multiple, including whole blood, swabs, tissue, and more
Sample input volume 50 μL–2 mL 50 μL–2 mL
Yield 1.5–4 μg (50 μL of blood)
2–12 μg (1 buccal swab)
1.5–4 μg (50 μL of blood)
2–12 μg (1 buccal swab)
Elution volume 50 μL–1 mL 50 μL–1 mL
Total processing time 6–96 samples in 30–45 min depending on sample type 6–96 samples in 30–45 min depending on sample type
Reactions (rxn) 1 kit = 100 rxn of 50–400 μL volume or
200 rxn of <200 μL volume;
bulk reagents sold separately
1 kit = 100 rxn of 50–400 μL volume or
200 rxn of <200 μL volume;
bulk reagents sold separately
Format Traditional Prefilled plates
Cat. No. A36570
Flex-ready: A36716
Duo-ready: A36584

FFPE Isolation

Sequentially isolate DNA and RNA from FFPE samples and get high-quality gDNA yields comparable to fresh frozen blood. Ideal for Oncomine Comprehensive and Oncomine Focus assays.

Sample workflow

Sample workflow

Recommended kit

  Applied Biosystems MagMAX FFPE DNA/RNA Ultra Kit
Applied Biosystems MagMAX FFPE DNA/RNA Ultra Kit
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Sample type FFPE tissue samples, such as resections, biopsies, and aspirates
Sample input volume 5–40 μm standard input of slides or curls
Elution volume 20–50 μL
Total processing time 20 min for DNA,
48 min for RNA
Reactions (rxn) 96 isolations (48 RNA and 48 DNA, or 96 DNA or 96 RNA)
Cat. No. A31881

Applied Biosystems AutoLys system

Speed up your deparaffinization process with the AutoLys system. Comprised of pretreated tubes and a rack system to reduce pipetting steps, the system eliminates the need for hazardous chemicals and minimizes loss of tissue sample.

Search AutoLys products

Note: Not included with the MagMAX FFPE DNA/RNA Ultra Kit.


Genetic testing

Speed up your processing of saliva samples for genetic testing. Recover DNA from 96 samples in 25 minutes that is compatible with NGS and array platforms. Reagents were tested with the 10 most common saliva collection devices to confirm consistent results.

Sample workflow

Genetic testing

Recommended kit

  Applied Biosystems MagMAX gDNA Saliva Kit
Applied Biosystems MagMAX gDNA Saliva Kit
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Sample type Fresh and preserved saliva
Sample input volume 200 μL–2 mL
Yield 100 ng/μL saliva on average (yield is dependent on each individual donor)
Elution volume 50 μL
Total processing time 25 min (5 min hands-on time)
Reactions (rxn) 100 or 500
Cat. No. A39059, A39060

Microbiome research

  • Recover from a wide range of microorganisms without additional processing steps
  • Enzyme-based digestion

Sample workflow

Microbiome research

Recommended kit

  MagMAX Microbiome Nucleic Acid Isolation Kit
MagMAX Microbiome Nucleic Acid Isolation Kits
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Sample type Stool, soil, swabs, urine, and saliva
Sample input volume 100 mg of stool, 250 mg of soil, 400 μL of biofluid
Yield 40–60 μg of stool, 5–10 μg of soil
Elution volume 50–200 μL or more
Total processing time 50 min for stool, 70 min for soil and biofluid
Reactions (rxn) 100
Cat. No. A42357 (with plates)
A42358 (with tubes)

Liquid biopsy

  • Rapid purification of nucleic acids from cell-free samples for liquid biopsy research
  • Elute in volumes compatible with Ion Torrent Oncomine assays
  • Optimized specifically for enrichment of cfDNA and cell free total nucleic acid (cfTNA), not gDNA, which means increased recovery and lower starting volumes

Sample workflow

Sample workflow

Recommended kits

  Applied Biosystems MagMAX Cell-Free DNA Isolation Kit
Applied Biosystems MagMAX Cell-Free DNA Isolation Kit
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Applied Biosystems MagMAX Cell-Free DNA Isolation Kit
Applied Biosystems MagMAX Cell-Free Total Nucleic Acid Isolation Kit
Learn more
Sample type Serum, plasma, and urine Serum and plasma
Sample input volume 0.5–10 mL plasma,
0.1–10 mL serum,
10 mL of urine
1–6 mL plasma
Yield 40–50 ng from 4 mL plasma Up to 50 ng DNA from 4 mL
and 5–100 pg from 10 mL of sample input
Elution volume 15–50 μL 15–60 μL
Total processing time 45–55 min total (10–15 min hands-on time) 45–55 min total (10–15 min hands-on time)
Reactions (rxn) 50 rxn for 2 mL plasma input
25 rxn for 4 mL plasma input
50 rxn for 2 mL plasma input
25 rxn for 4 mL plasma input
Cat. No. A29319
A36716

mRNA

Target and capture the mRNA transcriptome from an extremely wide variety of crude starting samples using Invitrogen Dynabeads mRNA DIRECT kits.

These kits are designed for simple and rapid isolation of pure, intact poly(A) RNA directly from the crude lysate of animal and plant cells and tissues. The isolated mRNA is suitable for use in all downstream applications.

  • Fast—15 minute procedure yields pure, intact mRNA
  • Highly pure mRNA isolation—upstream of cDNA synthesis
  • Sensitive mRNA isolation—enables cDNA synthesis and cDNA library construction from ultra-small starting samples

Recommended kits

  Dynabeads mRNA Purification Kit (for mRNA purification from total RNA preps)
Dynabeads mRNA Purification Kit (for mRNA purification from total RNA preps)
Learn more
Sample type Blood, cells, FFPE and fixed samples, liquid samples (e.g., serum), plant samples, RNA, tissue, viral samples, yeast
Sample input volume Up to 20 x 106 cells
2–200 mg animal tissue
4–400 mg plant tissue
Yield 2 μg of mRNA per 1 mg of Dynabeads oligo(dT)
Elution volume 10–100 μL
Total processing time 15 min
Reactions (rxn) 20 or 40
Cat. No. 61006

Plant DNA or RNA

Plant DNA or RNA Use the Applied Biosystems MagMAX Plant DNA and RNA kits for automation-ready purification of DNA and RNA from a wide variety of plant species.

  • Purification of total RNA from wide variety of plant species and tissue types, including plant fungus
  • Optimized for the isolation of DNA from 10–100 mg plant samples
  • Purified RNA is free of proteins, nucleases, and other contaminants or inhibitors

Recommended kits

 
MagMAX Plant DNA Kit and RNA Kit
  DNA RNA
Sample type Plant tissue
Sample input volume Up to 100 mg
Yield Varies based on starting material
Total processing time <40 min Up to 75 min
Reactions (rxn) 96 or 384
Cat. No. A32549A32580 A33784, A33899

RNA for gene expression

Isolate total RNA, as well as small RNAs such as microRNA (miRNA), from a wide variety of sample types using a phenol-free extraction (no organic extraction).

Sample workflow

Sample workflow

Recommended kit

  Applied Biosystems MagMAX mirVana Total RNA Isolation Kit
Applied Biosystems MagMAX mirVana Total RNA Isolation Kit
Learn more
Sample type Multiple, including plasma/serum, whole blood, tissue, cell culture, urine
Sample input volume 50 μL of blood,
up to 50 mg of tissue,
1 x 106 cells,
100 μL of plasma or serum,
250 μL of urine
Yield 100 ng RNA from whole blood (50 μL),
600 ng from 5 mg mouse brain,
2.5 μg from 5 mg mouse liver
Elution volume 50 μL
Total processing time 1.5–2 hr total time (20 min hands-on time)
Reactions (rxn) 96
Cat. No. A27828

Viral/Pathogen

  • Recovery—compatible with low titer samples
  • No carrier required—less interference with NGS and fewer steps required

Sample workflow

Microbiome research

Recommended kits

  MagMAX Viral/Pathogen Nucleic Acid Isolation Kit
MagMAX Viral/Pathogen Nucleic Acid Isolation Kit
Learn more
MagMAX Viral/Pathogen Ultra Nucleic Acid Isolation Kit
MagMAX Viral/Pathogen Ultra Nucleic Acid Isolation Kit
Learn more
Sample type Plasma, whole blood, urine, BAL, VTM, serum, CSF, and saliva Swabs, plasma, whole blood, urine, BAL, VTM, serum, CSF, and saliva
Microbes   Lysed virus, yeast, fungus, gram-negative and gram-positive bacteria
Sample input volume 200 μL–2 mL 200 μL–2 mL
Yield ≥50 copies ≥50 viral copies,
≥1,000 CFU (bacteria or fungi)
Elution volume 50–100 μL 60–100 μL
Total processing time 40 min 65 min
Reactions (rxn) 100 100
Cat. No. A42352
A42356

Immunoprecipitation (IP)

KingFisher instruments with Dynabeads magnetic beads offer the best balance of high yield and reproducibility with low nonspecific binding, which is one reason they have become the gold standard for immunoprecipitation using magnetic beads.

  • Automated IP in 40 min
  • High yield with low antibody consumption and low nonspecific binding
  • Rapid target binding; short incubation and separation time

Learn more

Sample workflow

Immunoprecipitation (IP)

Recommended kits

dynabeads-10006D-small

Peptide mapping and quantitation

Amazingly fast, simple, reproducible, and sensitive protein digestion with immunoaffinity capture, giving you higher confidence in your chromatography and liquid chromatography-mass spectrometry (LC-MS) results.

Peptide mapping workflow

Peptide mapping workflow

Recommended kits

  Thermo Scientific SMART Digest immunoaffinity kits for peptide quantitation
Thermo Scientific SMART Digest immunoaffinity kits for peptide quantitation
Learn more
Sample type Cell broth/plasma/serum
Sample input volume Up to 50 μL (200 pg–3.5 mg of total protein)
Total processing time Typical mAb ~45 min (dependent on size and complexity of protein)
Cat. No. 60109-10160109-109

Peptide quantitation workflow

Peptide quantitation workflow

Recommended kits

  Thermo Scientific SMART Digest immunoaffinity kits for peptide quantitation
Thermo Scientific SMART Digest immunoaffinity kits for peptide quantitation
Learn more
Sample type Cell broth/plasma/serum
Sample input volume Up to 50 μL (200 pg–3.5 mg of total protein)
Total processing time Typical mAb ~3–4 hr (dependent on size and complexity of protein)
Cat. No. 60110-10160110-104

Protein purification

Thermo Scientific Pierce magnetic agarose beads support a variety of ligands for IP, co-IP, pull-down, and other high-throughput affinity screening applications utilizing immobilized protein A/G, Ni-NTA, glutathione, and anti-FLAG.

  • High binding—up to 100-fold greater binding capacity than traditional magnetic beads
  • More choices—multiple ligands available for different purification strategies, including immobilized protein A/G, Ni-NTA, glutathione, and anti-FLAG
  • Automation-compatible—can be used with magnetic particle processors for higher-throughput applications
  • Flexible—can scale up or down as needed using adaptable protocol

Learn more

Sample workflow

Protein purification

Recommended kits