Organism TypeHamsterPrevious results showed that when apoB53 is stably expressed in CHO cells (B53 cells), it is rapidly degraded, producing an N-terminal fragment that is secreted without a lipid core . The proteolytic inhibitor ALLN blocks the formation of the N-terminal apoB fragment and causes intact apoB53 to accumulate as a transmembrane protein in isolated microsomes. These cells, stably transfected with a plasmid expressing rat 7-hydroxylase, are hereafter referred to as JF7 cells.
TissuesOvary
PhenotypeAdherent
Primaryyes
Application