Organism TypeHumanPrimary cultures of human cardiomyocytes were prepared by surgery requiring cardiopulmonary bypass. The human right atrial appendages were minced in small pieces and digested. Cells were collected and cultured in Iscove's modified Dulbecco's medium (Invitrogen) supplemented with 10% FBS, L-glutamine, and antibiotics.
TissuesHeart
PhenotypeAdherent
Primaryyes
ApplicationCell Culture / Growth Conditions