wit49 wilms tumor Cells | Thermo Fisher Scientific

Organism Type	Human	The WiT49 cell line was derived from a primary lung metastasis of an aggressive Wilms tumor.2 WiT49 cells were maintained in 1:1 high-glucose Dulbecco’s modified Eagle’s medium/ nutrient mixture F-12, 10% fetal calf serum, 100 units/ml penicillin, and 100 mg/ml streptomycin. Transfections were done using LipofectAMINE Plus . Stably transfected cells were selected with 1 mg/ml G418 (Life Technologies, Inc.) and maintained in 0.6 mg/ml G418
Tissues	Lung
Phenotype	Either
Primary	yes
Application	Cell Culture / Growth Conditions,Transfection: Gene Expression

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