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Organism Type | Human | The WiT49 cell line was derived from a primary lung metastasis of an aggressive Wilms tumor.2 WiT49 cells were maintained in 1:1 high-glucose Dulbecco’s modified Eagle’s medium/ nutrient mixture F-12, 10% fetal calf serum, 100 units/ml penicillin, and 100 mg/ml streptomycin. Transfections were done using LipofectAMINE Plus . Stably transfected cells were selected with 1 mg/ml G418 (Life Technologies, Inc.) and maintained in 0.6 mg/ml G418 |
Tissues | Lung | |
Phenotype | Either | |
Primary | yes | |
Application | Cell Culture / Growth Conditions,Transfection: Gene Expression |
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