Tetramethylrhodamine (TRITC)

Tetramethylrhodamine (TRITC) is a bright orange-fluorescent dye with excitation ideally suited to the 532 nm laser line. It is commonly conjugated to antibodies and proteins for cellular imaging applications.

Rhodamine phalloidin is one of the most commonly used fluorescent phalloidin conjugates, as evidenced by over 19,000 citations in the literature. We offer rhodamine reactive dyes as well as other protein and antibody conjugates or probes.

For brighter conjugates and higher photostability, we offer Alexa Fluor 555 as a reactive dye or conjugated to a variety of antibodies, peptides, proteins, tracers, or amplification substrates; all optimized for cellular labeling and detection.

Tetramethylrhodamine (TRITC) dashboard


Initial brightness

 
 
 
 
 
Tetramethylrhodamine is a traditional orange-fluorescent dye which has been used extensively to label protein conjugates such as phalloidins for cytoskeleton counterstaining or antibody conjugates for immunofluorescence.  
   

Photostability in buffer

 
 
 
 
 
   

Photostability in antifade

 
 
 
 
 

  488/532 TRITC 557 576  
 
Laser line

Common filter set

Excitation max

Emission max
 

Tetramethylrhodamine (TRITC) products

We offer a broad range of tetramethylrhodamine products including secondary antibodies, bioconjugates, and more.

See all TRITC Primary Antibodies
See all TRITC Secondary Antibodies

Tetramethylrhodamine (TRITC)

Zebrafish retina labeled with ELF 97 Immunohistochemistry Kit, Hoechst 33342, and tetramethylrhodamine wheat germ agglutinin.

Tetramethylrhodamine (TRITC) reactive dyes

Select dye-labeling chemistries for conjugation with the following reactive groups: 

High-performance alternatives to tetramethylrhodamine (TRITC)

showcase.par.64681.image.0.0.1

Reactive dyes and conjugates

The Invitrogen Alexa Fluor 555 dye—with spectral properties and quantum yield nearly identical to those of tetramethylrhodamine (TRITC) —produces brighter, more photostable conjugates. These conjugates are ideal for imaging and other applications requiring increased sensitivity and environmentally insensitive fluorescence detection.

We offer Alexa Fluor 555 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to allow you to generate your own antibody conjugates or probes.

Learn more about Alexa Fluor 555 dye

Phalloidin conjugates

Fluorescent conjugates of phalloidin using the Invitrogen Alexa Fluor dyes are designed for bright, specific F-actin staining for most applications across the full spectral range. The Invitrogen Alexa Fluor phalloidin conjugates provide researchers with fluorescent probes that are designed to be superior in brightness and photostability to all other spectrally similar conjugates tested.

Learn more about Alexa Fluor phalloidin conjugates

 

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.