Target cell structures and proteins of interest with organelle-selective dyes, stains, and primary antibodies
Labeling various targets with separate fluorescent colors allows you to visualize different structures or proteins within cells in the same sample. Ways to label your target fluorescently include fluorescent dyes, immunolabeling, and fluorescent fusion proteins—all of which provide a means to selectively mark structures and proteins within the cell and allow you to see them more easily when you image.
Many fluorescence tools for cell biology are essentially fluorophores that have been modified in different ways or conjugated to various molecules to give them a certain function or allow them to bind to specific organelles or proteins. Through chemical modifications, a single fluorophore can be produced in a number of variant forms, each with a different specificity. For example, the green-fluorescent Invitrogen Alexa Fluor 488 dye molecule can be modified to target actin filaments, can be attached to an IgG for use in immunolabeling, or can act as a whole-cell stain.
The Invitrogen portfolio offers more than 51,000 high-quality primary antibodies, with specificity to over 85% of the proteome. Some of these antibodies are attached directly to a broad range of intensely fluorescent markers and labels, including Invitrogen Alexa Fluor dyes.