Detecting complex biological assemblies requires maximum clarity of fluorescence signals and separation of signals from background noise. Standard immunofluorescence labeling rarely provides the best signal-to-noise visibility. The difference between producing a good and a great publication-quality image requires fine-tuning your sample’s signal for peak specificity, definition, and amplification.
Quickly and easily choose the labeling solution you need
High- to medium-abundance protein targets
Secondary antibodies are used for the indirect detection of target antigens. While primary antibodies bind directly to the target, secondary antibodies bind indirectly by using the primary antibody as a bridge to the targeted biomolecule. This methodology serves to amplify signals and increase sensitivity to maximize detection.
Explore secondary antibodies
Medium- to low-abundance protein targets
Streptavidin conjugates can increase the number of fluorophores that label your target, and boost their signals. Streptavidin-based amplification techniques are widely used in fluorescence imaging for improved sensitivity of detection with primary and secondary antibodies.
Find out more about streptavidin signal amplification for imaging
Low-abundance protein targets
For low-abundance protein targets that are not detectable by conventional means, tyramide signal amplification (TSA, PerkinElmer) provides sensitive detection without compromising resolution. TSA technology employs an enzyme that releases reactive dyes in the presence of hydrogen peroxide to bring targets out of the background with definition and clarity.
Learn more about imaging low-abundance targets with TSA